| Objective: Observe the expression of NK cell ligand MICA(majorhistocompatibility complex class Ⅰc hain-related protein A)contain solubleMICA in the epithelial ovarian cancer patients serum and membrane MICA inthe epithelial ovarian cancer tissue. Explore the correlation between theepithelial ovarian cancer patients serum and the expression of membraneMICA. Explore the relation between the expression of MICA and theepithelial ovarian cancer in the term of the different cell differentiation degreeand histology type and clinical stages. Provide theoretical targets for the earlydiagnosis and treatment on epithelial ovarian cancer patients.Methods:59cases of epithelial ovarian cancer patients serum and tissuewere selected, compared with12cases of epithelial ovarian benign tumor and15cases of normal ovaries epithelium tissue respectively. The serum levels ofMICA of peripheral blood were assessed by MICA-specific enzyme-linkedimmunosorbent assay. Immunohistochemical method (S-P technique) wasused to detect the distribution of MICA in epithelial ovarian cancer tissues. Touse SPSS13.0,SAS V8statistical analysis software analyze experimentaldata. p<0.05indicates that the difference had a statistical significance.Results:1ELISA: the date was (mean±standard deviation)1.1The serum levels of sMICA in59cases of epithelial ovarian cancer were(20.79±8.37) ng/ml,in12cases of epithelial ovarian benign tumor were(9.98±1.75) ng/ml,in15cases of health donors were (6.47±1.69) ng/mlrespectively. The serum levels of sMICA in epithelial ovarian cancer were thehighest one, the benign were the middle one and the health donors were thelowest one. The difference between them had statistical significance(Chi-Square statistics value was55.315,p=0.000, p﹤0.05). 1.2The serum levels of sMICA of epithelial ovarian cancer in21cases of thepoorly differentiated patients were (24.59±10.20)ng/ml,in38cases of themoderately differentiated patients were (18.70±6.39) ng/ml. The serum levelsof sMICA in the poorly differentiated patients were significantly higher thanthat in the moderately one (Mann-Whitney U statistics value was251.500,p=0.020,p<0.05).1.3The serum levels of sMICA of epithelial ovarian cancer in11cases ofstage Ⅰ～Ⅱwere (14.10±2.17) ng/ml,in48cases of stage Ⅲ～Ⅳ were(22.33±8.52) ng/ml respectively,The serum levels of sMICA in stage Ⅲ～Ⅳwere significantly higher than that in stage Ⅰ～Ⅱ(Mann-Whitney U statisticsvalue was88.000,p=0.001,p﹤0.05).1.4The serum levels of sMICA in different histological type of epithelialovarian cancer were19cases of endometrioid cancer (21.10±9.84) ng/ml,21cases of serous cancer (20.35±7.97) ng/ml,11cases of poorly differentiatedcarcinoma (20.27±6.81) ng/ml,8cases of mucinous cancer (21.95±8.99)ng/ml.There were no obvious difference in different histological type ofepithelial ovarian cancer (Chi-Square statistics value was0.162,p=0.983,p>0.05).2Immunohistochemical method (S-P technique): the positive expression ofmMICA situate at cell membrane and cell cytoplasm. On the basis of XuLiangzhong score three points were the positive one.The expression ofmMICA at cell membrane and cell cytoplasm occupy all the observation ofthe number of percentage was the positive expression rate.2.1The positive expression rate of mMICA in epithelial ovarian cancer tissuesand in benign tissues were57.63%(34/59)and8.33%(1/12) respectively,Inthe15cases of normal oarian epithelial didn't express. The difference betweenthe mMICA positive expression rate in epithelial ovarian cancer tissues andthat in benign tissues had a statistical significance (Chi-Square value was9.694,p=0.002,p<0.05).2.2The expression rate of mMICA was68.42%(26/38)in the moderatelydifferentiated ovarian cancer tissues while38.10%(8/21)in the poorly differentiated. Compared the mMICA expression in the poorly differentiatedwith that in the moderately differentiated, the difference had a statisticalsignificance(Chi-Square value was5.094,p=0.024, p<0.05). In poorlydifferentiated carcinoma, MICA mainly shedding at cancer interstitial cell.So,most poorly differentiated carcinoma score less three points, mMICApresent a negative express.2.3The expression rate of mMICA in stage Ⅲ～Ⅳ was higher than that instage Ⅰ～Ⅱ,There were50.00%(24/48) and90.91%(10/11) respectively,The difference had a statistical significance (Continuity Adj.Chi-Square valuewas4.573, p=0.033, p<0.05). In stage Ⅲ～Ⅳ, MICA mainly shedding atcancer interstitial cell. So,in most stage Ⅲ～Ⅳ cancer score less threepoints, mMICA present a negative express.2.4The expression rate of mMICA in different histological type of epithelialovarian malignant tumor were in endometrioid carcinoma57.89%(11/19),inserous carcinoma52.38%(11/21),in poorly differentiated carcinoma63.64%(7/11),in mucinous carcinoma62.50%(5/8).There were no obvious differencein different histological type of epithelial ovarian malignant tumor(p=0.931,p>0.05).3The correlation between the positive expression rate in certain group cancertissue which score three points and the serum levels of sMICA incorresponding patients. The positive expression was mMICA offer the yellowparticles in cell membrane and cell cytoplasm. Selecte the positive cellsexpression rate average value in3slices and5highpower fields.3.1The pearson correlation coefficient between the positive cells expressionrate in11cases of stageⅠ～Ⅱcancer tissues and the serum levels of sMICAin corresponding patients was-0.638,p=0.047,they were present a negativecorrelation and had a statistical significance.3.2The pearson correlation coefficient between the positive cells expressionrate in24cases of stage Ⅲ～Ⅳcancer tissues and the serum levels of sMICAin corresponding patients was-0.762,p=0.000,they were present a negativecorrelation and had a statistical significance. 3.3The pearson correlation coefficient between the positive cells expressionrate in26cases of moderately differentiated ovarian cancer tissues and theserum levels of sMICA in corresponding patients was-0.757,p=0.000,theywere present a negative correlation and had a statistical significance.3.4The pearson correlation coefficient between the positive cells expressionrate in8cases of the poorly differentiated ovarian cancer tissues and the serumlevels of sMICA in corresponding patients was-0.726,p=0.041,they werepresent a negative correlation and had a statistical significance.3.5The spearman correlation coefficient between the positive cells expressionrate in34cases of the ovarian cancer tissues and the serum levels of sMICA incorresponding patients was rs=-0.428,p=0.011,they were present a negativecorrelation and had a statistical significance.3.6There was no linear correlation in different histological type of epithelialovarian cancer tissues with the serum levels of sMICA in correspondingpatients. The correlation coefficient and p value were：in11cases of positiveendometrioid cancer the spearman correlation coefficient rs=-0.270,p=0.422；in11cases of positive serous cancer the pearson correlation coefficient r=-0.385, p=0.242；in7cases of positive poorly differentiated the carcinomapearson correlation coefficient r=-0.557,p=0.194；in5cases of positivemucinous cancer the spearman correlation coefficient rs=-0.300,p=0.624respectively.Conclusions: The levels of sMICA in the epithelial ovarian cancer serumand the positive expression rate of MICA in epithelial ovarian cancer arecorrelated with the tumour character, the cell differentiation and clinical stage.There was no correlation in different histological type of epithelial ovariancancer tissues. The poorer cell differentiated and the advanced stage,the highlevel of sMICA but the low positive expression rate of mMICA. In poorlydifferentiated carcinoma and in stage Ⅲ～Ⅳ cancer, MICA mainlyshedding at cancer interstitial cell. So,it score less three points, mMICApresent a negative express.There present a negative correlation between thepositive cells expression rate and the serum levels of sMICA in corresponding patients In stageⅠ～Ⅱ andⅢ～Ⅳ,in moderately and poorly differentiatedovarian cancer tissues,in the all ovarian cancer tissues. There was no linearcorrelation in different histological type of epithelial ovarian cancer tissueswith the serum levels of sMICA in corresponding patients.sMICA may play a critical role in tumor immunological escape inepithelial ovarian cancer. To observe the expression of MICA on the epithelialovarian cancer serum and tissue can help the clinical assistant diagnosis.sMICA could be considered as one of the tumour symbol theoretically.Thesignificance of detecting mMICA and sMICA may be of important clinicalvalue in analysis the development of disease and immune function condition. |
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