Ultrasonic Radiation Poria, Lotus Seed Polysaccharide Structure Characterization

Semen Nelumbinis and Poria crude polysaccharides were extracted from hot water, the water temperature is90℃, the extraction time is3h and the solid to liquid ratio is1:20. Three polysaccharide components of Semen Nelumbinis were made,named SN1SN2and SN3; and also obtained Poria polysaccharide named PCM. Deal with the polysaccharide sample using ultrasonic radiation, to compare the structure before and after ultrasound, reveals that the ultrasonic degradation effect on water-soluble polysaccharide, in order to reveal the internal mechanism of the ultrasonic radiation degradation of the polysaccharide, provided the theoretical data and experimental basis of ultrasonic degradation of polysaccharide structure onto the industrialized The main research contents and the corresponding experimental results can be summarized as follows,(1)Extraction and purificationA water soluble polysaccharide from Semen Nelumbinis was obtained by hot water, and precipitated by ethanol. Three polysaccharide fractions (SN1, SN2, SN3) were separated from crude polysaccharide using cetyltrimethyl ammonium bromide(CTAB) precipitation, boric acid complex, and SephadexG-150column. By that method we also obtained PCM polysaccharide samples. Protein content were as follows:SN1protein content is27.2%, SN2protein content is36.4%, SN3protein content is22.0%and the PCM protein content is6.1%. The Semen Nelumbinis polysaccharide has antioxidant activities of the hydroxyl radicals and superoxide anion radicals, scavenging increased with polysaccharide concentration increasing, reducing power and scavenging DPPH free radical capacity is weak.(2) Semen Nelumbinis polysaccharide structure analysisSemen Nelumbinis polysaccharides chemical compositions were determined in this study by gas chromatography, infrared spectroscopy, ultraviolet spectroscopy. The results show that SN1mainly composed of glucose, galactose, including a-D-glucopyranosyl ring; SN2may have glucan as main chain, and contains4other monosaccharides; SN3has7monosaccharides, suggesting that amino acid is a polysaccharide or proteoglycan. Periodate oxidation and Smith degradation results show that polysaccharide SN1and SN2has the1→3,1→2or1→4and1→(end). Atomic force microscope observe polysaccharide SN1appear the spiral distribution and SN2appear the aggregate distribution.(3)The glucan morphology before and after ultrasonic radiationWe use400W power ultrasound treatment on glucan, observe the surface morphology of samples before and after the ultrasound with atomic force microscopy. Results show that the ultrasonic degradation affects of the polysaccharide chain, making the branch structure of the polysaccharide reduced.(4)Degradation of polysaccharides by mono-frequency ultrasonic and momosaccharide composition,infrared spectroscopy and circular dichroism analysisTwo polysaccharides of different structures were degradated by mono-frequency ultrasonic. Gas chromatography, infrared spectroscopy and circular dichroism were used to analysis the sample structures before and after degradation. It is found that ultrasound do not change the characteristic structures of polysaccharide. The content of mono-saccharide changes, and the hand of the characteristics have no change. It is also found that after ultrasound treatment Poria polysaccharide has blue shift.