Effect Of Lentivirus Mediated Transfer Of Mfv Ⅱ/FC Gene On Growth Of Prostate Carcinoma Xenografts In SCID Mice

Objective To evaluate therapeutic potential of fusion gene mFVⅡ/Fc for humanprostate carcinoma of severe combined immunodeficient (SCID) mouse model.Methods Human prostate carcinoma mice model was created by using Pc-3pros-tate cancer cells.When average volume of tumor was about100-150mm~3,eigh-teen SCID mice with human prostate carcinoma were randomly divided intotherapeutic group,negative control group and blank control group (n=6),separatelyreceiving intratumoral injection with mFV Ⅱ/Fc/Lentivirus(1x108TU/0.2ml/time),null Lentivirus(1x108TU/0.2ml/time) and NS (1x108TU/0.2ml/time). During thetreatment period,growth of prostate carcinoma xenografts was observed.The micewere sacrifi-ced after42days,and tumor masses were removed.Tumor volume andtumor weight were measured,CD34antigen was evaluated by immunohistochemistryto observe intratumoral microvessel density.Results mFVⅡ/Fc/Lentivirus could significantly inhibit thegrowth ofprostate carcinoma of SCID murine. Tumor volume and weight decreased signify-cantly in treatment group,compared with negative control group and blank controlgroup(P<0.01).The treatment group also had much lower intratumoral microvesseldensity compared with other two groups(P<0.01)Conclusions Lentivirus mediated transfer of mFV Ⅱ/Fcgene could inhibit thegrowth of prostate carcinoma xenografts of SCID mice and also decrease the intratu-moral microvessel density, improve the tumor inhibition rate.