| Objective: Since aetiology and pathophysiology of pre-eclampsiaremains poorly understood. The renin-angiotensin system (RAS) is anendocrine system that ie responsible for blood pressure, sodium, and fluidhomeostasis. Although the underlying mechanisms remain unclear, there isgrowing evidence to suggest that dysregulation of the local and circulatingRAS may be involved in the pathophysiology of pre-eclampsia. The changesin the expression of Ang-(1-7) and ACE2during pregnancy should beimportant for the pathophysiological aspects of pre-eclampsia. The presentstudy is to investigate the expression leves of Ang-(1-7) and ACE2in maternalblood, placental tissue and cord blood of normal pregnant women andpre-eclampsia patients. To explore the roles of Ang-(1-7) and ACE2inpathogenesis of pre-eclampsia. Expect to provide new ideas for clinicaldiagnosis and treament of pre-eclampsia.Methods: Three separate groups were included. Group1(n=30, normalpregnant women). Group2(n=30, mild pre-eclampsia patients, MP). Group3(n=30, sever pre-eclampsia patients, SP). Maternal peripheral blood,placental tissue and cord blood specimens were collected to examined.Ang-(1-7) and ACE2were measurement by ELISA. All the data wereanalyzed by SPSS13.0software．Results：1Ang-(1-7) expression levels in maternal serum were gradually increased insevere pre-eclampsia group, mild pre-eclampsia group and control group. Thedata were (288.76±49.97)ng/L,(344.48±50.07)ng/L and (406.78±58.35) ng/L.There was significant difference. Ang-(1-7) expression levels in cord serum ofthe three groups were gradually increased. The data were (448.19±94.73)ng/L, (543.72±81.84)ng/L and (631.95±85.18) ng/L. There was significantdifference. The expression levels of Ang-(1-7) in placental tissuehomogenate of the three groups were (82.95±5.35)ng/,(80.98±6.51) ng/L and(79.94±7.22)ng/L, it showed no significant difference.2ACE2expression levels in maternal serum were gradually increased insevere pre-eclampsia group, mild pre-eclampsia group and control group. Thedata were (103.70±17.57)U/L,(119.27±21.39)U/L and (142.01±22.07)U/L.There was significant difference. ACE2expression levels in cord serum inmild pre-eclampsia and sever pre-eclampsia were (200.50±18.79)U/L and(204.17±16.34)U/L, it respectively and significantly lower than those incontrols (215.93±23.19)U/L (P <0.05). But there was no significantdifference between MP and SP. The expression levels of ACE2in placentaltissue homogenate of the three groups were (34.02±2.27)U/L,(33.19±1.88)U/L and (32.88±1.90)U/L, it showed no significant difference.3The expression levels of Ang-(1-7) in cord serum of severe pre-eclampsiagroup,mild pre-eclampsia group and control group were significantly higherthan those in maternal serum [(448.19±94.73)ng/L vs (288.76±49.97)ng/L;(543.72±81.84)ng/L vs (344.48±50.07)ng/L;(631.95±85.18)ng/L vs(406.78±58.35)ng/L;(P <0.05)]. The expression levels of ACE2in cordserum of the three groups were significantly higher than those in maternalserum [(204.17±16.34)ng/L vs (103.70±17.57) ng/L;(200.50±18.79)ng/Lvs(119.27±21.39) ng/L;(215.93±23.19)ng/L vs (142.01±22.07) ng/L(P <0.05)].4The expression levels of Ang-(1-7) in maternal serum, placental tissuehomogenate and cord serum were positively correlated (r1=0.393, P <0.01；r2=0.941, P <0.01；r3=0.348, P <0.01). Positive correlations were found ofACE2expression levels in maternal serum, placenta tissue homogenate andcord serum (r4=0.383, P <0.01；r5=0.734, P <0.01；r6=0.480, P <0.01).5Positive correlation were found between Ang-(1-7) and ACE2in maternalserum, placental tissue homogenate and cord serum (r7=0.920, P <0.01,r8=0.498, P <0.01,r9=0.742, P <0.01). 6Ang-(1-7) expression levels in maternal serum of severe pre-eclampsiagroup and mild pre-eclampsia group were negatively correlated with SBP(r10=-0.462,P <0.05; r11=-0.441, P <0.05) and DBP (r12=-0.407,P <0.05;r13=-0.693,P <0.05). Positive correlation were found between Ang-(1-7)levels in cord serum of severe pre-eclampsia group, mild pre-eclampsia groupand birth weight (r14=0.604, P <0.01; r15=0.436, P <0.05).Conclusions：1Ang-(1-7) and ACE2has a significant positive correlation in maternalserum,cord serum and placental tissue of pre-eclamptic patients and normalpregnant women.2Ang-(1-7) and ACE2levels were significantly lower in maternal serum andcord serum of pre-eclamptic patients than normal pregnant women. Ang-(1-7)and ACE2levels are related to the occurrence and development ofpre-eclampsia.3The circulating RAS and the local RAS play important roles in thepathophysiology of pre-eclampsia. |
PDF Full Text Request