DREB2E Participate In Arabidopsis Adapt To A Variety Of Abiotic Stress

Abiotic stresses such as drought, high salinity, and cold are common adverse environmental conditionsthat significantly influence plant growth and productivity worldwide. The phytohormone abscisic acid（ABA） plays an important in physiological and developmental responses as well as in coordinating variousstress signal transduction pathways in plants. Abiotic stress gene expression is regulated by the different oftranscription factor. Recently studies have demonstrated that a lot of transcription factors, such asMYB-（R1） R2R3, AP2/EREBP, WRKY and other transcription factors, have an important role in theregulation to plant response in different stress. Some studies have reported that DREB family transcriptionfactor, such as DRREB2A, DREB2B and so on, participated in the abiotic stress response widely, such asABA, drought and high salt, low temperature. In previously research, we found that ABA and reactiveoxygen treatment can induce changes in DREB2E gene expression, but it plays an important role aboutabiotic stress adaptation has not been reported.In order to further research DRREB2E gene function, the research constructed DREB2Eover-expression transgenic positive lines and RNAi transgenic positive lines. We use this materials andobserve materials seedling growth and root growth phenotype in the different of abiotic stress conditionssuch as ABA, H₂O₂, NaCl, mannitol. We found that DREB2E over-expression transgenic positive lines andRNAi transgenic positive lines have obviously difference compared with WT. Abiotic stress such as ABA,H₂O₂, NaCl, mannitol have an effect on DREB2E over-expression transgenic positive lines and RNAitransgenic positive lines germination rate, cotyledon growth, root length and number, so we guess thatDREB2E genes involved in arabidopsis plant adapt to a variety of abiotic stress response widely. And then we observed that the DREB2E over-expression transgenic positive lines temperature islower than the wild type, and water loss rate and stomatal are higher than the wild type, and stomatal, waterloss rate and temperature have no obviously difference in RNAi transgenic positive lines compared with thewild type, so we analysis that the DREB2E gene regulate the stomatal movement.At the same time, we obtain transgenic positive lines of DREB2E GUS, we analyzed the DREB2Egene expression in the different tissues of arabidopsis, we found that the DREB2E gene are expressed in theyoung plant’s root, leaf, flower, and the DREB2E gene are not expressed in the mature plant’s stem andfruit pod.We also detected gene expression level about the ABA downstream relation by semi-quantitativeRT-PCR, We found that RD29B gene has knocked down in DREB2E RNAi plant too, which indicated thatthe transcription factor DREB2E maybe has related to the RD29B in the stress condition.We also have detected DREB2E gene and RD29B gene about ABA and H₂O₂stress time bysemi-quantitative RT-PCR, we found that DREB2E gene and RD29B gene have increased along with theABA treatment time. But DREB2E gene has declined and RD29B gene has not changed along with H₂O₂treatment time. It indicated that DREB2E genes and RD29B gene involved in the ABA signal transductionpathways.From the above of all experiments, we concluded that DREB2E gene widely participate in abioticstress response, DREB2E maybe regulate RD29B and other downstream genes, DREB2E involved inABA-dependent transcriptional regulation and abiotic stress response process.