The Study Of Histology And Endocrine Cells In The Digestive Tract Of Salamandrella Keyserlingii

Histology observation slowed that the digestive tract during postembryonicdevelopment of Salamandrella keyserlingii had a spatial and temporal order: thedigestive tract organs was according to stomach, intestine and esophagus of developingsequence appear.The digestive tract of the wall was according to the mucous epithelium,inner muscle, external muscle, adventitia, lamina propria, submucosa and muscularismucosa of developing sequence appear. The digestive tract of the adult ofSalamandrella keyserlingii had the four layers: mueosa, submueosa, muscular layer andadventitia.The silver nitrate method of Grimelius showed that argentaffine cells were firstappeared between mucous epithelial cells in the stomach of Salamandrella keyserlingiiat stage41. The argentaffine cells were first observed in the intestine at stage42. Theargentaffine cells were appeared in the esophagus most night at stage43. But, theargyrophil cells could be found throughout the digestive tract of the adult ofSalamandrella keyserlingii. The distribution density was the highest in rectum, followedby pylorica part of stomach and ileum and the lowest in stomach. And the shape of theargentaffine cells were mainly round, ellipse, cone-shape and shuttle-shaped. Accordingto the morphology of the argentaffine cells, we suggest that argentaffine cells in thedigestive tract of Salamandrella keyserlingii have endocrine, exocrine and paracrinefunctions.Immunhistochemical observation showed that5-HT positive cells distributethroughout the digestive tract of Salamandrella keyserlingii. The distribution densitywas the highest in esophagus, followed by pylorica part of stomach and the lowest induodinum. SS cells were observed throughout the digestive tract except in cardiac partof stomach, jejunum and rectum. The distribution density of SS cells was the highest inpylorica part of stomach and the lowest in ileum. Gas cells distributed mainly induodenum and jejunum. Glu cells distributed mainly in cardiac part of stomach and thebody of stomach. SP cells were observed only in the body of stomach. But, PP cellswere not detected.The shape of the endocrine cells in the digestive tract of Salamandrel- la keyserlingii were mainly round, ellipse, cone-shape and shuttle-shaped.Between thedistributive density of five kinds of endocrine cells are adapt with physiologicalfunction of digestive tract.