The Analysis Of Metabolites From Actinoalloteichus Nanshanensis Sp.Nov. Neau119

In order to discovery new active compounds, a good antibacterial activity bacteria Actinoalloteichus nanshanensis sp. nov. NEAU119, which was identified as a novel Actinoalloteichus, was studied, including extraction, isolation, structure identification and bioactivity determination.The experiment used the antitumor model of human lung cell line A549in vitro and antibacterial model against Staphylococcus epidermidis and Candida albicans to screen the optimum fermentation medium for the NEAU119. Finally, we chose the XG (Glucose2.0%, Malt Extract1.0%, Corn soluble amylum1.0%, Yeast Extract0.4%, CaCO30.2%and pH7.2-7.4) as the optimum medium. Then the fermentation was carried out in a fermenter (50L). With the use of silica gel, LH-20and HPLC, the isolation of the fermentation broth was taken on.12compounds were finally obtained, and their structures were elucidated on the basis of MS and NMR spectroscopic analysis.3compounds were macrocyclic lactam compounds containing a5,5,6-tricyclic skeleton and a tetramic-acid unit. The compound2was new, and compounds1and3were known compounds. All of the other9compounds (4-12) were all new with a cyclopentane unit. The antineoplasmic and antibacterial activity of11compounds were measured, and compounds1,2,3and8showed good inhibitory activity against the growth of human lung adenocarcinoma cell line A549with the IC5o values of1.71,2.37,1.71and14.67μg mL-1, respectively. Besides, compounds8and12showed good inhibitory activity against the growth of human leukemia cell line K562with the IC50values of11.87.92.03μg mL-1, and of humanrenal cell carcinoma cell line ACHN with the IC50values of23.36,97.60μg mL-1, respectively. And compounds1,2and3showed good antibacterial activity.The10new compounds were named as:4-dehydroxy dihydromaltophilin (2);4-(3-hydroxypropyl)-6,7-dihydro-2H-cyclopenta[c]pyridine-1,5-dione (4);2-(hydroxymethyl)-6-(3-hydroxypropyl)-2-methoxy-3,3a-dihydro-2H-cyclopenta[b]furan-4(6aH)-one(5);(Z)-2-(1,5-di-hydroxypent-2-en-2-yl)-3-(hydroxymethyl)cyclopent-2-enone (6);(E>2-(1,5-dihydroxypent-2-en-2-yl)-3-(hydroxylmethyl)cyclopent-2-enone (7);2H-2-(2-hydroxyethyl)-4-(3-hydroxypropyl)-6,7-dihydro-cyclopenta[c]pyridine-1,5-dione (8);4R*,7R*,11R*,2H-3,4,4a,5a,7,8-hexhydro-4-hydro-xy-pyra-no[2,3-b]cyclopenta[d]pyran-9(6H)-one (9);4R*,7S*,11S*,2H-3,4,4a,5a,7,8-hexhydro-4-hydroxy-pyrano[2,3-b]cyclopenta[d]pyran-9(6H)-one (10);7R*,11R*,2H-3,4,4a,5a,7,8-hexhydro-pyrano[2,3-b]cyclopenta[d]pyran-9(6H)-one (11);2H-3,5a,7,8-hexhydro-pyrano [2,3-b]cyclopenta [d]pyran-9(6H)-one(12).