The Study Of Measuring Hydrogen Exchange Rate Without Spin-Lattice Relaxation Time With NMR

Hydrogen exchange is a hot topic of research in the field of biologgical NMR (Nuclear Magnetic Resonance) all the time. It is a very crucial type of exchange reactions of biochemistry, especially in the studying of solution, structure and function of biological macromolecules. It is a hot spot and a difficult issue to study hydrogen exchange rate in NMR.At present, studying of hydrogen exchange with NMR has become a primary approach to study dynamics of biological macromolecules. Unfortunately, during the exchange process the relaxation also affects the decay. There is no method to eliminate the impact of spin-lattice relaxation time so far. This problem is a key point of the hydrogen exchange rate measurement accuracy.In this paper, we will study biological molecules hydrogen exchange with NMR and develop a new method for measuring hydrogen exchange rate. First of all, we design a new pulse for measuring hydrogen exchange rates. Then, we develop a theoretical equation between the hydrogen exchange rate k and spin-lattice relaxation rate R(R=1/T1), M(t)=[R/(R+k)]*{1-exp[-(R+k)t]}. From the method of least square fitting, both the spin-lattice relaxation time and hydrogen exchange rate are derived. Furthermore, we build a simulation solution of hydrogen exchange process models. The amide signal acquired by the HSQC sequence increases accordingly the experiment results and computer simulations. It can be seen the theoretical results matches well with the experiment results. The eventual establishment of a hydrogen exchange rate measurement research method.In this thesis, we design a new method of measuring hydrogen exchange rate with NMR. This method can eliminate the effects of spin-lattice relaxation, compared with the previous studies. It brings a great of significance to the studying of biological macromolecules hydrogen exchange.