Study On The Microflora And Enzyme Activities During Germination Of The Malting Barley In Gansu Corridor

The dynamic variation patterns both of epiphytic microflora and main hydrolases activityduring two key production chains-the barley material and germination in malting ofprocessing enterprise were studied,by using malting barley produced in Gansu Corridor as theresearch object.Advantage bacterial phases were separated and identified by using classicalmethod of microbial purification to investigate the microflora and hydrolase activity dynamicvariation laws and interactions between them in order to provide the theory basis andtechnological support for improving domestic malt quality. It had research value and realisticsignificance. The main conclusions obtained were as follows:1. The epiphytic bacteria colony count on beer barley yielded from three typical areas inGansu Corridor was the largest, reached106cfu/g-107cfu/g; the yeast count was104cfu/g-105cfu/g; and the mould count was103cfu/g-105cfu/g, which changed greatly fromdifferent origins. Nine dominate strains of bacteria, nine dominate strains of yeasts and elevendominate strains of moulds had been separated and identified from epiphytic microflora ofbarley.2. The amount of epiphytic microorganisms on material during germination were higherthan malting barley.The dominant species of epiphytic microflora were different frommaterial during germination to malting barley.3. During germination, the amount of epiphytic microorganisms on material increasedThe variation laws of microbial groups at different material layers were different. Thedominant species of epiphytic microflor changed with barley germination.Themicroorganisms on the equipment surfaces had an impact on epiphytic microorganisms onmaterial during germination.4. In the epiphytic microflora on material during germination, the dominant bacteria wereGluconobacter oxydans, Klebslella Pneumoniae and Aeromonas hydrophila. the dominantyeasts were Breiianomyces custersii,Saccharomy kluyverl,Candida zeylanoidcs,Pichiaheimii,Pichia ohmerl. the dominant molds were Mucor silvaticus and Geotrichum candidum.5. The main hydrolase activity during germination increased rapidly, increasing patternsof hydrolase activity had differences at different material layers; besides of,the hydrolase activity were related not only to the malt growth but also to the water,temperature,concentration of carbon dioxide/oxygen and so on in material.6. The colony count of bacteria had positive correlation with activities of amylase,protease, xylanase andβ-glucanase. Pearson correlation coefficients were0.86、0.834、0.867、0.917（P＜0.01）respectively; the colony count of fungus had positive correlation withactivities of amylase, xylanase andβ-glucanase. All of pearson correlation coefficientsreached0.85. The interaction between the colony count of fungus and activities of proteasewas low and not significant.