Ecl Study And Analytical Application Of Ru（Bpy）₃²⁺ And Rhb

This dissertation is intended to discuss an analytical application method on Electrochemiluminescence (ECL), introduce the background of chemically modified electrode and development and application of ECL system on carbon nanotube modified electrode. ECL and application of RhB and Ru(bpy)32+were studied.Firstly, bare glassy carbon electrode, single-walled carbon nanotube physically modified electrode and RhB chemically modified SWNT coating electrode were employed as working electrode to investigate ECL of RhB, which was applied in the determination of sodium sulfide. It was found that a linear response for sodium sulfide concentration from1.0×10-16～1.0×10-11mol L-1with a remarkable detection limit of1.0×10-16mol L-1at RhB-hexamethylenediamine-SWNT modified glassy carbon electrode. Compared with the ECL of RhB on multiple-walled carbon nanotube modified glass carbon electrode for the determination of sodium sulfide (linear response from6.0×10-10～1.0×10-8mol L-1with a detection limit of2.0×10-10mol L-1), ECL method of RhB-hexamethylenediamine-SWNT gave lower detection limit and the broader linear. The proposed method was applied to the determination of1.0×10-15mol L-1sodium sulfide with good reproducibility and stability. All these provided possibility to develop a novel ECL detection method on sodium sulfide.Secondly, ECL of Ru(bpy)32+at graphite oxide and graphene modified glassy carbon electrode has been studied and was applied for melamine and MG. The lowest detection limit of melamine and MG at graphite oxide modified glassy carbon electrode is1.0×10-16mol L-1and3.0×10.15mol L-1respectively, which has been proved to be a useful method with good reproducibility and stability.Thirdly, ECL of Ru(bpy)32+at glass carbon (GC) electrode has been employed for determination of hydeazine hydrate at first time. For Ru(bpy)32+, it gave a linear response for hydeazine hydrate concentration from1.0×10-9～1.0×10-5mol L-1with a remarkable detection limit of1.0×10-9mol L-1in pH9phosphate buffer. The proposed method has good reproducibility and stability. All these provided possibility to develop a novel ECL detection method on hydeazine hydrate.Lastly, ECL efficiency of Ru(phen)32+and Ru(bpy)32+were compared and Ru(phen)32+ECL efficiency was higher. Meanwhile, Ru(phen)32+was applied in dectecting melamine. The lowest detection limit of melamine was1.0×10-11mol L-1and the detection range was1.0× 10.11~6.0x10"7mol L-1. Compared with detecting melamine by ECL of Ru(bpy)32+, this method was more accurate and sensitive. The lowest detection limit of MG was1.0*10-10mol L-1and the detection range was1.0x10-10-6.0x10-6mol L-1with good reproducibility and stability.