The Research On Extraction Technology Of Polysaccharide And Flavonoids From Pteris Vittata And Their Antibacterial Activity

Pteris Vittata is a kind of pteridophytes, the whole plant or rhizome can be used for medicine, can be taken throughout the year. It is used for flu prevention, dysentery, rheumatism, pain, bruises. Flavonoid is a more of chemical components contained in the pteridophytes, has strong biological activity, and is widely used in the pharmaceutical and food additives. Studies on Pteris Vittata remain in the field of environmental protection, the reports about purification and functional are very few. In this paper, we chose Pteris Vittata as raw material, studied on extraction, separation and purification of flavonoids aglycones and polysaccharose as well as antibacterial activity in vitro.1. The preliminary experiment of chemical composition in Pteris Vittata. The extract of Pteris Vittata was detected through ultraviolet spectrometer; the UV spectrogram was similar to isflavones. The structure was determined by joining displacement reagent of measured ultraviolet spectrum,4on a hydroxyl,3-OH and A, B ring were no two adjacent phenol group.2.Polysaccharides were extracted by the methods of water extraction and ethanol precipitation,the optimum extraction technology parameters determined by experiments are followed:the extraction temperature90℃,material-water ratio1:25extraction time4h. In above conditions the extraction yield of polysaccharide was3.78%.Infrared spectrum research found the polysaccharide in Pteris Vittata had the characteristics of general polysaccharides material absorption peaks, and at the same time in the structure of the existing pyranoid ring.3. The fungus ferment produce cellulose enzyme of two kinds of fermentation way: liquid fermentation and solid fermentation was studied and compared. The results show that: Aspergillus niger is suit for Solid-State Fermentation producing cellulose enzyme, the best cultivation condition is that:straw powder and bran quality for6:4, adjusting the initial medium pH4.0,20%inoculated quantity, the enzyme activity of CMC is3.47U/ml,and the enzyme activity of FPA is2.34U/ml With the enzyme thick liquid in mold fermentation, yield of flavonoids form Pteris Vittata effectively improved. The extraction technology conditions is that:according to1:40of additives and mold the thick liquid ferment produce enzymes to centipede grass extraction solvent, from50℃and the extraction time is1h.4.The studied on flavonoids aglycone purification Pteris Vittata.We chose from four kinds of macro porous resin and through the comparison of static adsorption to screen the suitable kind for the flavonoid aglyeone purification Pteris Vittata, and studied on the static adsorption, desorption characteristics of this resin and determined the dynamic adsorption and desorption process. The results show that theD101resin has a higher adsorption and desorption rate than others, so it was suitable for purifying flavonoid from Pteris Vittata.The best conditions of adsorption and desorption were as follows:pH value was4.5, sample flow rate was1ml/min;eluate was ethanol solution of60%, eluate flow rate was1ml/min. The liquid concentration sample for4.02mg/ml, after purification, the concentration up to14.71mg/ml, achieved good purification effect.5. Then the antimicrobial activity of flavonoids to several kinds of microorganism and the stabilities of flavonoids to pH value, metal ion, and temperature and UV radiation were also researched. The result showed that:The flavonoids, isolated from the Pteris Vittata by water, ethylacetate,1-butanol, and the three different flavonoids could inhibit the growth of a series microorganism, especially the Staphylococcus aureus effectively, but they have no effect on Escherichia coli, aspergillus and mycete. Determined result of the MIC of flavonoid from Pteris Vittata extracted by ethylacetate of Staphylococcus aureus Staphylococcus aureus, Vibro parahaemolyticus was2.443,4.886,4.886mg/ml. The MBC was2.443,4.886,4.886mg/ml respectively. Determined result of the MIC of flavonoid extracted by1-butanol of Staphylococcus aureus, Staphylococcus aureus was5.064,10.127mg/ml. The MBC was5.064,10.127mg/ml respectively. Determined result of the MIC of flavonoid extracted by water of Staphylococcus aureus, Staphylococcus aureus, Vibro parahaemolyticus was5.663,5.663,5.663mg/ml. The MBC was5.663,5.663,5.663mg/ml respectively.The stabilities of the different flavonoids to pH presented distinct inhibitory effects. It could facilitate the antimicrobial activity of flavonoids at weak alkaline pH of7.5. Metal ion(Fe3+) had indistinctive effect on the antimicrobial activity of flavonoids extracted by1-butanol and water. Metal ion (Ca2+) had obviously inhibitory influence of flavonoids on Vibro parahaemolyticus, extracted by water. Ultraviolet could eliminate distinctly the antimicrobial activity of flavonoids. Although, the short-time ultraviolet had limitedly effect on the antimicrobial activity of flavonoids, and continued ultravioleting could undoubtly weaken the antimicrobial activity of flavonoids.There was no antibacterial effect of polysaccharide from PterisVittata.