Preparation Of Molecularly Imprinting-Carbon Nanotubes Electrode And It S Selective Determination Of Chloramphenicol

Antibiotic misuse has give rise to the increasingly ubiquitous antibiotic resistant bacteria around the globe. Chloramphenicol (CAP) was one of the oldest artificial broad-spectrum antibiotics, it’s residual in environment has posed a serious threat to both human and animals. The traditional developed analytical methods for CAP determination in domestic and aboard include chromatography and immunoassay technique, etc.. nevertheless the above methods have the limitation of requiring complicated pretreatments. large piece of equipments and long response time. The electrochemical sensor are relatively fast and sensitive, while has no selectivity. Considering the requirements of the on site detection and the complexity of the environment samples, a novel technique for the rapid, sensitive and selective determination of CAP is urgent.In the present study, we combined the advantages of the predetermined recognition property of molecularly imprinting polymers (MIPs), and the large specific surface area and the excellent electron transfer properties of carbon nanomaterials and metal nanoparticles. We fabricated the MIP film on the nanomaterials modified Ti plate electrode and glassy carbon electrode (GCE) by thermalpolymerization and electropolymerization method, respectively. The resulted novel MIP electrochemical sensor exhibited a excellent sensitivity and selectivity for the determination of CAP in aqueous solution. The electrochemical determination was proposed by differential pulse voltammetry (DPV).The properties of the imprinted electrode were characterized and its application in real water samples was evaluated. The results confirmed that the nanomaterials increased the electro transfer rate and enhanced the sensitivity of the modified electrode, and the MIP film provided a selective recognition property. Under the optimized condition, the imprinted electrode exhibited a linear range of0.1～100mg/L, with a limit of detection of0.024mg/L (S/N=3) for CAP detection. When penicillin-G (Pen-G), thiamphenicol (TAM) and p-nitrophenol (p-NP) were used as the interferents, the imprinted sensor showed good selectivity and interference immunity, and the signal responses of Pen-G, TAM and p-NP were0.11%,5.8%and10%that of the response for CAP, respectively. The present research developed a novel CAP determination method in aqueous solution by combining the advantages of the molecularly imprinting technique, carbon nanomaterials and electrochemical technique. The proposed method provide a technical support for the high sensitive, selective and on site antibiotics determination platform in environment, and is important for the accuracy and rapid Environmental Risk Assessment as well as the Environmental Pollution Emergency Warning Systerm.