Toxicity Effects Of Kinds Of Typical Nano-particles On A549Cells By Nuclear Analytical Techniques

TiO₂nano-materials, graphene and its composites are the hot, representative and typicalnanometer materials, with a large scale application. TiO₂nanopaticles are in the form of anataseand ruile. Because of its special physical and chemical properties, TiO₂nano-materials arealreadly widely used in paints, comestics, water pollution treatment, catalyst, electronic material,medicine, biomedicine and so on. Graphene and its composites are the spotlight in scientific fieldand they have become the most popular materials in recent years. There is much research valueand many potential applications prospect in the information field, the energy field, devicefabrication and biomedicine. It’s very necessary that we should research the interactions betweenthese kinds of typical nano-materials, for its being likely to the best chance in disease diagnosis,treatment and drug development.Our study uses the molecule detection techniques, electron microscopy technology,synchrotron radiation technology, and discusses systematically and roundly the biocompatibility,cytotoxicity, possible mechanism of two crystal phase of TiO₂nanopaticles, grapheme and itscomposites in the level of cell and molecule. The results have implications for the biomedicalapplication and safety assessment. The primary results of study are as follow:1. Using the molecule detection techniques, it’s obvious differences of cell membrane damageand mitochondria damage to A549cells because the crystal phase of TiO₂nanopaticles isdifferent.2. In the morphology, the anatase TiO₂nanopaticles mainly existe around the cell membrane asagglomerates of dozens of nanometers after administration to A549cells and there are no obviousdamage to the cell membrane. Then most of the rutile TiO₂nanopaticles merge with cytoplasm asagglomerates of hundreds of nanometers.3. The results of spectroscopy analysis shows that two crystal phase of TiO₂nanopaticles canbind to some components in cells after interaction, but there are no obvious effect on theintracellular site with different crystal phase.4. GO shows good biocompatibility and easily enter the cells without causing any serious damage to them.5. TiO₂separated from the grapheme component of G-T after administration to A549cells. Thecytotoxicity of G-T is similar to that of TiO₂and may have been caused by oxidative stress.