The Study On Rapid Detection Of Veterinary Drugs In Livestock Products Using Bioluminescent Bacteria

Veterinary drug residues in livestock products not only bring potential hazards to the health ofconsumers, but also influence the sustainable development of the livestock industry. At present, the fast,accurate and cheap detection technology of veterinary drug residues in livestock products are being studiedat home and abroad. In the paper, on the basis of principles of bioluminescent bacteria, V.Qinghaiensis-Q67and P.phosphoreum was used as toxicity indicator strains to study ten common veterinary drugs’action rules and acute toxicity by determining its relatively luminescence intensity under differentveterinary drug concentrations and its EC50to the ten veterinary drugs; Second general rotation test wereused to study combined toxicity of the mixture of Quinolones in livestock produces by mixing uptwo,three,four kinds of Quinolones on V.Qianghaiensis to reveal Quinolones’ whole and interactive toxiceffect; Based on the detection of veterinary drug in the water using V.Qianghaiensis-Q67, the action rulesand acute toxicity of Enrofloxacin and Sulfamonomethoxine Sodium in pork extract were studied.The aimwas to detecting veterinary drugs in livestock products rapidly and evaluated the rapid safety of livestockproducts.The main conclusions are drawn as followings:1.That the effects of10kinds of veterinary drugs on V.Qinghaiensis-Q67was the same asP.phosphoreum-T3, the action rule was that the lower the veterinary drugs’ mass concentration, the strongerthe fluorescence. According to the action rules, it was observed that V.Qinghaiensis-Q67can detectQuinolones trace as low as0.002mg/L. Sulfamonomethoxine Sodium trace was detect as low as0.1mg/Lby P.phosphoreum-T3.2.Within certain rang of concentration, the relative luminescence intensity of V.Qinghaiensis-Q67anddifferent veterinary drug concentrations presented good linear relationship, the coefficients ofdetermination were from0.7911to0.9890. The order of ten veterinary drug toxicity on V.Qinghaiensis-Q67was: Furazolidone(4.08mg/L)>Nitrofurazone (5.21mg/L)>Levofloxacin (91.991mg/L)>Norfloxacin（210.788mg/L）>Sulfa six SDM sodium(476.835mg/L)> Sarafloxacin(704.486mg/L)>Sulfadiazine Sodium(871.196mg/L)> Enrofloxacin(1219.104mg/L)> Ciprofloxacin(1795.098mg/L)>Sulfamonomethoxine sodium(4454.516mg/L). The liner ranges of Enrofloxacin and SulfamonomethoxineSodium were from250to2000mg/L and from3to24mg/L, the liner relationships were observed betweentheir mass concentration and the relative luminescence intensity of P.phosphoreum-T3. And the toxicity ofSulfamonomethoxine Sodium on P.phosphoreum-T3was higher than that of Enrofloxacin.3.That combinations of different Quinolones with different concentration showed distinct toxicityeffect. Nine regression models on Quinolones concentration and relative fluorescence ofV.Qianghaiensis-Q67were derived by Second general rotation test,and the coefficients of determinationwere from0.8919to0.9976. At the same time, The combined toxicity of the mixture of Quinolones onV.Qianghaiensis-Q67was complicated, which was related with mass concentration and drugs species.4.When the liner ranges of Enrofloxacin and Sulfamonomethoxine Sodium in pork extract were from0.01to2500mg/L and from0.03to3000mg/L, the relative luminescence intensity of V.Qianghaiensis-Q67is negatively related to veterinary drugs’ mass concentration, with the coefficients ofdetermination of0.907and0.9985. It was observed that V.Qinghaiensis-Q67can detectSulfamonomethoxine Sodium in pork extract trace as low as0.1mg/L, so that V.Qinghaiensis-Q67canmeet the detection requirement of Sulfamonomethoxine Sodium in livestock products.