Construction Of Phosphorylcholine Coating Through Layer By Layer Self-Assembly Methods

Much attention has been paid to the surface-modification of the cardiovascular implant materials. Phosphorylcholine (PC) coatings have been fabricated via two different strategies by layer-by-layer self-assembly method.Layer by layer self-assembly of poly[2-(methacryloyloxy) ethyl phosphorylcholine] multilayer via the ionic complexation with zirconiumZirconium-phosphonate (Zr-P) ionic complexation chemistry is explored as a new approach to fabricate poly[2-(methacryloyloxy) ethyl phosphorylcholine](PMPC) multilayer film by layer-by-layer self-assembly method. Quartz crystal microbalance with dissipation (QCM-D) and optical ellipsometry measurements demonstrated that PMPC layer can be fully absorbed on each Zr4+layer. The thickness of the multilayer film with a good linear relationship was followed by the ellipsometry in situ adlayer characterization. The influence of pH of the PMPC and Zr4+solutions on the multilayer deposition were investigated by optical ellipsometry. QCM-D results indicated that the multilayer film is stable in a PBS flowing chamber at a high flow rate of5.2×10-3m/s. The ellipsometry data demonstrated that67.2%of the film still remained on the silicon wafer after being strong shaken in PBS at80rpm for12hours. The adsorption of bovine serum albumin (BSA) and fetal bovine serum (FBS) on the PMPC surface was monitored by the QCM-D and spectroscopic ellipsometry, and the results showed the multilayer film have excellent protein resistance.Covalently bonded layer-by-layer assembly of polymer multilayer film based on epoxy groupsThe covalent bond multilayer coating which contains PC has been formed based on the reaction of epoxy groups and primary amine groups. The layer by layer self-assembly of P(MPC-GMA)(MG) and PEI was carried out by spin coating method. The growth of the multilayer from MG and PEI was successfully traced by QCM-D and optical ellipsometry measurements. The thickness of the multilayer film with a linear relationship was followed by the ellipsometry in situ adlayer characterization. The multilayer films [MG/PEI]n were stable in PBS and water has been demonstrated by strong shaken in PBS and water at80rpm for80hours. The influence of reaction time of epoxy groups and primary amine groups was also carried out. Biomacromolecules also be used to layer by layer self-assembly with MG.