Preparation Of Multi-Amine Oxidized Cellulose And Adsorption Capacity For Bilirubin And Lead Ion

Liver that play an important role in the synthesis, detoxification, metabolism, secretion, biotransformation, and immune defense is one of the vital organs of the body. If the liver was severely compromised, leading to the extensive necrosis of liver cells, these processes would be hindered, resulting in jaundice and hyperbilirubinemia. When there is too much bilirubin in the blood to be excreted, producing severe toxic effects on the body. In addition, the excessive content of heavy metal in the body will put more burden on the the metabolism of the liver and kidney. Blood perfusion is one of the effective methods to remove bilirubin and heavy metal ions in the body to treat the patients with liver failure or biliary obstruction. In this paper three kinds of adsorbents(Diethylenetriamine diadehyde cellulose, triethylenetetramine diadehyde cellulose, hexamethylene diamine diadehyde cellulose) for blood perfusion were prepared with microcrystalline cellulose as carrier by three steps of chlorination, amination and oxidation. These products were characterized by FTIR、XRD、CPMA13CNMR and Kjeldahl s methods. Optimum conditions of synthesizing triethylenetetramine cellulose and hexamethylene diamine cellulose were studied by determining the nitrogen content. Their adsorption properties to bilirubin were studied and then the better adsorbents(TETA-DAC and HDA-DAC) for bilirubin were chosen to determine the adsorption properties to heavy metallic ion Pb2+. The corresponding mechanism of adsorpting bilirubin and Pb2+were discussed.The influence of the reaction temperature, reaction time and molar ratio of amino to chlorinated cellulose on the products were studied by nitrogen content. Optimum conditions of synthesizing TETA-C and HDA-C were determined by single factor. Result showed that the synthetical conditions of TETA-C were obtained as molar ratio of amino to chlorinated cellulose7.5:1, reaction temperature100℃and reaction time12hours. The synthetical conditions of HDA-C were obtained as molar ratio of amino to chlorinated cellulose7.5:1, reaction temperature100℃and reaction time14hours. The nitrogen content of corresponding TETA-C and HDA-C were4.25%and6.65%respectively. Finally, the hydroxyl groups at the C-2and C-3positions were oxidized selectively into a dialdehyde with periodate sodium. The aldehyde content of DETA-DAC, TETA-DAC and HDA-DAC were79%,77%,73%respectively.The adsorption property for bilirubin were determined under the condition of simulating the human environment. DETA-DAC reached adsorption equilibrium of bilirubin after about2.5hours and the max adsorption capacity to bilirubin was39mg/g. DETA-DAC reached adsorption equilibrium after about2hours and the max adsorption capacity to bilirubin was44mg/g. HDA-DAC reached adsorption equilibrium after about1.5hours. When the initial concentration of BR up to300mg/L, the amount of BR adsorption on HDA-DAC reached to51.58mg/g. The effect of pH, ionic strength, temperature and the BSA concentration on the adsorption capacity of DETA-DAC, TETA-DAC and HDA-DAC for bilirubin were investigated in a batch system. The experimental data indicate that the adsorption capacity of these adsorbents increased with the increase of temperature, but decreased with the the increase of ionic strength and the BSA concentration. The adsorption capacity of TETA-C and HDA-C synthesized under different conditions also were determined in a batch system. The results showed that the the amount of BR increase with the increase of amine content. The adsorption properties after reusing the adsorbents were investigated through regenerated experiments. The results showed that the three kinds of adsorbents still remained good adsorption capacity after the first regeneration and regeneration effect of TETA-DAC was better than others.The adsorption property of TETA-DAC and HDA-DAC to Pb2+was determined by static adsorption experiments and the effect of adsorbents content and temperature on the adsorption capacity also been determined. The adsorption of TETA-DAC and HDA-DAC to Pb2+reached equilibrium after5hours and3hours respectively. The amount of Pb2+adsorption on TETA-DAC and HDA-DAC reach to22.5mg/g and9.9mg/g respectively. the adsorption isotherm of Pb2+on TETA-DAC fit well with Freundich equation. In addition, the increase of adsorbent concentration play a negative role in the adsorption of Pb2+, while the increase of temperature favor adsorption of Pb2+.