A Research On Microbial Diversity Of Different Flooding In Daqing Oil Field

With the rapid technological development of Microbial Enhanced Oil Recovery (MEOR),it has demonstrated a good view and attracted more global attentions.While the advantages of this technology is still far from totally performed.One of the main reasons is the lack of Knowledge on the microbial communities and their functions. The purpose of this dissertation is to determine the application range of MEOR and to supply theories for Pilot test. Then, the evaluation and the molecular detection of microorganism in Oil reservoirs of different flooding.The methods based on16S rDNA,which were mainly Real-time PCR and clone librabry approaches,were Performed to acquire the species and heritage diversity of reservoir microorganism.The following conclusions were gained by analyzing quantitatively and qualita-tively microorganism in the samples and calculating some important parameters1.According to the result of the Real-time PCR,the numbers of the bacterial in the oil and the produced water were ranged from2.33×105～2.94×107and4.39×106～1.90×107, respectively,while the numbers of the archaeal in the oil and the produced water were ranged from1.1×103～1.28×104and5.88×103～2.12×105.The numbers of the bacterial and the archaeal in the water were higher than those in the oil.2.The clone libraries of bacterial showed that uncultured Rheinheimera sp. And uncul-tured bacteria were the main species in the SYH,while Pseudomonas sp.,which can degrade paraffin,remove sulfur and produce surfactant to lower viscosity,were the main species in the GAO and53.3.The clone libraries of archaeal showed that there were widespread methanogens (such as Methanoculleus, Methanosaeta and Methanobacteria.) and uncultured archaeon in the oil samples. It suggests that methanogens might be involved in the biodegradation of crude oil and the Methanogenesis.