The Study Of Tyrosine Fluorescence Properties

Detections of the amino acids have great significance in the cycle ofmarine carbon and nitrogen. The results of detection is influenced easilythrough traditional amino acids analyzer because of long-time processing.The three-dimension fluorescent spectrum is widely used in the study ofmarine fluorescent substances because of high selectivity, high informationand so on. The fluorescent properties of fluorescent substances is not onlyrelated to the structure itself, but also the environment, such as pH,hydrostatic pressure and meta ion. Therefore, the study of fluorescentproperties of tyrosine in different environment is very necessary indetections of marine amino acids.The dissertation presents the research status of three-dimensionalfluorescence spectra. The experimental program and analytical methods arepresented.19groups of experimental program are designed on studyingfluorescent properties of tyrosine which investigated the influence of pH,metal ions and hydrostatic pressure.Results showed:（1）There are three fluorescent peaks in the three-dimensionalfluorescent spectra of tyrosine solution, including the peak of Rayleighscattering, the first characteristic peaks at Ex230nm and the secondcharacteristic peaks at Ex280nm.（2）The fluorescent intensity of tyrosine solution got the maximumvalue at pH6.0with the increase of pH from3.0to9.0. （3）The fluorescent intensity of tyrosine solution was quenched byCu²⁺and Ni²⁺,while no change by Mg²⁺. The quenching become strongerwhen the concentration of metal ion increased. The quenching weakenedwith the decrease of pH.（4） The fluorescent intensity of Tyr aqueous solution enhanced withincreased hydrostatic pressure. The quenching of Cu²⁺and Ni²⁺weakenedwhen the pressure increased from0.1MPa to60MPa.The study of tyrosine fluorescence properties provide some referencevalue for the on-line research of the ocean dissolved protein and aminoacids and the study of Tyrosine as exogenous or endogenous probes toexplore protein conformational changes and also provide a new idea onexploring the mechanisms of deep-sea hydrothermal vents communityresistant to high metal ion concentration.