Isolation Of Rare Actinomyces And Identification Of A Novel Actinomyce

With the continuous development and use of actinomycetes, rare actinomyces have gradually become a good source to produce antibiotics. Many researchers have turned to look the separation of rare actinomyces. This experiment is designed to extract from the soil to many kinds of fungi and bacteria plant pathogens having inhibition of rare actinomyces, and to strain crude extractings fermented liquid on antibacterial activity determination, looking for strains source to produce new antibiotics, and to form a special strains were identified.In this experiment, three kinds of separation medium were adopted.(add nalidixic acid20mg/L and cycloheximide50mg/L), respectively GYEA medium, the LSV-SVF medium and HV medium. Five different screening methods were used to make a separation of a total of9soil samples taken from Heilongjiang Province, Inner Mongolia, Guangdong Province,102actinomycetes isolated and obtained. The crude extract of fermentation broth of actinomycetes plate method for inhibiting bacterial activity, the experiment obtained16bacteria Micrococcus luteus and Bacillus spp. Inhibition zone diameter above15mm, of which four strains of the above two bacterial inhibition zone diameter were above25mm, respectively, N1(1), N2(5) the M2horses, and A1, and three strains of Micrococcus luteus inhibition zone was up to30mm, respectively. N1(1), N2(5) and A1; using cross method, the six kinds of fungi experiment within102actinomycetes inhibition was conducted, which showed that27bacteria had a significant inhibitory effect on at least two kinds of fungi and seven bacteria had a significant inhibitory effect on five species of fungi, respectively, N1(1), N1(5), H2small, H3small, M2Ma, A1and A4.The6Micromonospora bacteria Section description very similar to strains obtained from Micromonospora separation, respectively strains M2and C2, N1, G2,1-15and L5, were sequenced by the16S rRNA and in a preliminary contrast of sequencing results with the16S rRNA sequences in GenBank, where C2M2, G2and1-154bacteria similar to the highest strains were the Micromonospora chokoriensis strain2-19(6), which had a Micromonospora chokoriensis strain2-19(6) T similarity to C2(99.7%), M2(99.7%), G2(99.5%) and1-15(99.2%), but they the ISP3medium colony morphology was significantly different, thus we concluded that these four strains may be different. Strains L5and published Micromonospora bacteria Branch strains16S rRNA sequence similarity was relatively low, with similar maximum strain Micromonospora olivasterospora MK-70T (98.38%), Jishengella endophytica202201T (98.35%), Micromonospora rhizosphaerae211018T (98.11%), Micromonospora yangpuensis FXJ6.011T (98.04%). Strain L5its highest similarity with bacteria the Micromonospora olivasterospora MK-70T in the phylogenetic tree was not on the same branch, but on the same branch as the new genus Jishengella endophytica202201T, therefore we concluded that strain L5may be a new species in the Jishengella endophytica genus or a new genus. In this experiment, phenotypic and physiological and biochemical level of L5were examined, and subsequent experiments will make study into its chemotaxonomic and molecular biological identification.