The Study Of Poly Cyclic Aromatic Hydrocarbons And Their Metabolites Pollution Level In Eco-environment Of Bohai Bay

Polycyclic aromatic hydrocarbons, abbreviation as PAHs, the main source is pyrolysisand incomplete combustion of organic matter, widely distributed in atmosphere, solid andwater environment, most of them are generally accepted carcinogens. Moreover, carrybiology metabolization action, some polycyclic aromatic hydrocarbons metabolites (PAHm)also still have carcinogenic in the environment. Therefore, to the comprehensive evaluation ofhuman exposure levels of risk of PAHs, the biological monitoring indicator of polycyclicaromatic hydrocarbons and their metabolites research has become one of the focus at homeand abroad.In this paper, using accelerated solvent extraction, separation by SPE and GC-MSanalysis, determination of the trace persist organic pollutants (polycyclic aromatichydrocarbons and their metabolites) in environmental and organisms, and the distribution ofits various organs in vivo were studied. The research is explored on separation and detectionconditions of PAHs and PAHm that the results are satisfactory. The recoveries were PAH74-120%；PAHm-OH95.3±13.2%；PAHm-CH387.3±6.4%.The research on distribution characteristics in different organs and pollution levels ofPAHs, PAHm-OH and PAHm-CH3in variety of fishes from the Beitangkou, Qikou,Gaoshaling, Dagukou and Demonstration area in west coast of Bohai Bay. And analyzed thesources, distributions and component characteristics of interstitial water and sediment thatfrom Beitangkou, Qikou, Dagukou. Pilot study of the environmental behavior, toxicequivalent and risk assessment of polycyclic aromatic hydrocarbon contaminants in theecosystem of the Bohai Bay, the results showed that the Dagukou had the highest level ofPAHs and PAHm in the Bohai Bay area, the content of low-ring PAHs was much higher thanthe high-ring PAHs, preliminary analysis of the chief sources of pollutants in this area wereindustrial pollution and oil combustion products, and compound pollution of living coal, gas,charcoal and other fuel combustion were also exist. The distribution trend of PAHs andPAHm pollutants in the whole ecosystem as a carnivores> zoobenthos> sediment>herbivores> water, this showed the cumulative effect of PAHs and PAHm by food chainobviously.The total concentrations range of PAHs, PAHm-OH, and PAHm-CH3in sediments andinterstitial water form the Bohai Bay were0.012-60.26ng/g,0.008-0.346ng/g,0.112-0.179ng/g, respectively, mostly were limited to low-ring PAHs (≤4rings), and the contents of PAHm were lower than PAHs. The pollution levels of PAHs and PAHm in sediments of threeintertidals followed by Dagukou> Qikou> Beitangkou, and Dagukou> Beitangkou> Qikou ininterstitial water. The Qikou pollution level was very close to the Beitangkou, but Dagukouwas much higher, that said, the pollution of Dagukou was more serious.The total concentrations range of PAHs, PAHm-OH, and PAHm-CH3in marineorganisms form the Bohai Bay were27.798-3953.7ng/g,0.039-282.89ng/g,0.295-34.178ng/g, respectively, mostly were limited to low-ring PAHs (≤3rings), but the4rings PAHsin the digestive gland and gonads of male Rapana venosa, and stomach and internal organs ofmulletare were also very high. In the five intertidal zones, the contamination level of PAHs inwere ordinal be: demonstration area> Dagukou> Beitangkou> Gaoshaling> Qikou; thedistribution trend of PAHs in the same species was intestine> liver> eye> gill> muscle>spleen> stomach> head; the9-phenanthrol pollution levels between organisms followedmullet from Beitangkou> synechogobius hasta from Beitangkou> Synechogobius hasta fromQikou> anchovy from Beitangkou> Mantis shrimp from Qikou, the distribution trend of9-phenanthrol in the same species was intestine> eye> liver> muscle> gill> stomach> spleen>head; and the9,10-dimethylanthracene pollution levels between organisms followed mulletfrom Beitangkou> Synechogobius hasta from Qikou> Mantis shrimp from Qikou> anchovyfrom Beitangkou> synechogobius hasta from Beitangkou, the distribution trend of9,10-dimethylanthracene in the same species was spleen> gill> eye> liver> stomach>intestine> muscle> head. As a conclusion, the pollutants content in digestive system of theseorganisms is higher than other parts.The risk of PAHs in sediments of Bohai Bay evaluates suggested that the hazardquotients of PAHs were less than1in all sediment samples, thus the PAHs in sediments couldnot yet cause adverse effects on biological. The toxicity equivalence analysis of biologicalsamples showed that7kinds of strong carcinogenic PAHs accounted for the major proportion,The greatest contribution was BaP (average31.36%). The toxic equivalence of five samplingpoints followed by demonstration area> Dagukou> Gaoshaling> Beitangkou> Qikou; throughobserve species sensitivity distribution of organisms to seven kinds of strong carcinogenicPAHs, they were slightly less than the Expose concentration distribution (ECD> SSD), thegreatest contribution of ECD was BaP (average62.4%), so because of the strong carcinogenictoxicity of the seven kinds of PAHs, the levels of risk in the environment depends on theexposure.