Fluorogenic And Chromogenic Fluorescein Spirolactam Based Probe For NO

Nitric oxide(NO) plays an important role in many physiologicalprocesses of the biology. It is an important mediator for the pathologicalconditions of various diseases, such as carcinogenesis, inflammation, andsignal transduction. Therefore, if the organisms living cells steady-statelevel of nitric oxide disorders, it will lead to serious disease. In order toexplore the mechanisms by which NO mediates various physiological andpathophysiological processes, the development of a method capable ofdetecting NO in biology has been an intriguing challenge for chemist.Thus, effective fluorescent probes for NO should feature avorablecompatibility with biological samples, low toxicity, and resistance tooxidation, visible-wavelength excitation and emission profiles to minimize sample damage and native cellular autofluorescence, and a turn-on orratiometric fluorescence response for mapping spatial resolution.This paper designed several fluorescent probes based on thespirolactam(nonfluorescent) to ring opened amide(fluorescent) ofrhodamine derivatives.First, we designed and synthesized a fluorogenic and chromogenicfluorescein spirolactam based probe for NO. We study the reactionmechanism and optimized the detecting condition. It is a non-fluorescentcompound, after treatment with NO, generates the fluorescent product.The new probe has high selectivity and sensitivity in detecting NO.Second, we synthesized two novel fluorescein spirolactamderivatives based on the first system in a simple way and raising theoutput largely. We then applied it to the detection of NO and optimized thedetecting condition.Third, we synthesized2’,7’-difluorfluorescein spirolactam diacetateand then applied to determine NO in macrophage cells.