Study On Preparation Methords Of Silk Fibroin Scaffolds With Highly Ordered Pores Structures

Silk is one of the animal fiber used early by human being, it is mainly applied inthe field of textile clothing. In recent years, following the advance of science, theapplication of silk have already extended into the field of food, toiletry, biomedicine,industry material and environmental protection.Regenerated silk fibroin prepared form Bombyx mori silkworm has been provedthat it adopted as scaffolds materials for tissue engineering due to its remarkablebiocompatibility, biodegradability and unique mechanical property. As we known, thepore size and microstructure of a scaffold not only play an important role in celladhesion, migration, and differentiation, but also for the exchange of nutrients andmetabolite wastes. How to control precisely the pore sizes and interconnectivity ofporous structures of a scaffold is still a problem.In this thesis, different particle size of polystyrene (PS) and polymethylmethacrylate (PMMA) microspheres are used to construct a ordered template. Finally, aordered porous silk fibroin scaffold was fabricated successfully through the infiltrationof regenerated silk fibroin solution and dissolution of microsphere template.Furthermore, we also study on the effects of the related parameters on the order porousstructure of silk fibroin. This can provide certain technical support for development andapplication of high performance biomaterials.The main results of this dissertation as following. In this study, PMMAmicrospheres with diameter ranging from98to122μm and PS microspheres in535nm,3μm,40μm and130μm was used to construct the ordered template forcontrolling the pore size of3-D silk fibroin porous scaffolds. By controlling theconglutination degree between the microspheres in template, the interconnectivity of theporous structures was controlled. The results show that the ordered porous structure and interconnectivity of silk scaffold can be controlled exactly by ordered microspherestemplate.