Investigation On The Preparation Of PA6/PVA Composite Nanofibers And Immobilized Enzyme

Electrostatic spinning principle is to make the polymer solution with charge or melt in electrostatic field of flow and deformation, then by solvent evaporation or melt cooling and curing, so get fibrous material. Electrostatic spinning fibre products mainly are non-woven fabric shape, for great specific surface area, and excellent mechanical properties and thermoelectric conductivity, filter protection materials, enhanced in composite materials, biological medicine such as the drug release, etc have broad application prospects. Now, electrostatic spinning has become the preparation of ultrafine fiber and nanoscale fibers of the important method.The molecular structure of the enzyme senior outside factors are very sensitive, such as hot, strong acid, strong alkali, organic solvents and other is not quite stable, and, in the reaction system in recycling free enzyme is very difficult, easy cause product pollution made product purification difficulties, which greatly improve the production cost, etc. The immobilized enzymes to a certain extent to solve the above problems. Compared with free enzymes, the stability of the immobilized enzyme increases, can use repeatedly, easy and reactant apart, be helpful for the separation and purification of products, so as to improve the quality of products. Immobilized enzyme in the biological medicine, biological engineering, environmental protection, etc of the broad research and application prospect.Constructing the carrier of excellent performance is often the key to success for immobilized enzyme. This topic to formic acid as solvent, with electrostatic spinning method for PA6/PVA composite nano fiber membrane, and will be used for the enzyme immobilization carrier. Through to the PA6/PVA blending ratio and spinning process parameters adjustment prepared the form good PA6/PVA composite nano fiber membrane, using infrared spectrum and surface tension determine instrument on the compatibility and hydrophilic characterized and analysis, and the scanning electron microscopy (sem) of nanometer fiber membrane morphology analysis. Using epichlorohydrin activation nano fiber membrane method material, using acetylcholinesterase fixed cellulose enzyme, and discusses in detail the enzyme immobilization conditions on the influence of the immobilized enzyme activity. Finally, the free enzyme and the nature of the immobilized enzyme comparison. Conclusions are as follows:(1) in the spinning voltage for19kv, spinning distance for20cm, silk fluid flow rate (0.2ml/h, under the condition of PA6the best blended with PVA than12%:4%. In this blended than, fiber with good outlook. PA6and is a pair of good compatibility PVA polymer. PA6/PVA composite nanofiber relative to the pure PA6, PVA nano-fibre, it has sex and membrane hydrophilic improved, effectively overcome the pure yarn PVA in aqueous solution system nano-fibre excessive swelling problem, has the good form stability.(2) using the activation of the conditions for:with NaOH and dimethyl and for mixed solvent were DMSO, activation temperature for60℃, activation time for4h, pH value of10, NaOH concentration of1.0mol/L. In these conditions activation nano fiber membrane materials. When add enzyme quantity of15mL, pH value is4.0and coupling time for5h, immobilized enzyme all reached the biggest energy than.(3) immobilized enzyme optimum reaction temperature65℃, the optimum pH value of4.0,0.7301Km micro-sphere constant mmol/L, the biggest Vmax reaction rate is0.4529μmol/min, and free of enzyme micro-sphere constant for0.4592Km mmol/L, the biggest Vmax reaction rate is0.5045μ mol/min, explain enzyme was fixed with the substrate after the affinity of weak, enzyme activity reduced. Immobilized enzyme thermal stability, repeated use stability and storage stability are improved.