The Detection And Toxicology Experiment Research Of3-chloro-1,2-propanediol-ester

Chloropropanol esters have become new potential risk factors in edible fatsand oils, which could release free chloropropanol esters on the effect of intestinalpancreatic lipase. Chloropropanol is a well-known food contaminants withpotential carcinogenicity which can reduce sperm and drop sperm activity of rat, itcan also inhibit male hormone and weaken the reproductive ability.In recent years, it has been reported abroad that3-MCPD-esters can be testedin fats and oils, while it is not found in domestic field. Based on the foreign studies,the experiment makes detection on different series of edible oils on3-MCPD-esterscontents and discusses cause of formation, to find whether the content could bereduced through the processing improvement in fats and oils.The results ofdetection is that, the content of3-MCPD-esters in different edibles can be listedfrom high to low orderly as camellia oil, hair rapeseed oil, level4rapeseed oil,olive oil, Chinese prickly ash oil, soybean oil, level2cotton oil, level1rapeseedoil, rapeseed oil, sesame oil to palm oil. The assessment scope is1250-51250μg/kg. And we know that the temperature of deodorization in refining technologyinfluence is the main reason for the formation of3-MCPD-ester.Because of the high content of3-MCPD ester have been tested in oils, theexperiment takes Ames test, MTT, bone marrow micronucleus test and spermdeformity test to study its toxicity.3-MCPD-esters is precursor of3-MCPD, and atpresent the detection on3-MCPD-esters is by converting it to3-MCPD. Accordingto German federal risk assessment (BfR) and international life science society(ILSI) reports that, the toxicology evaluation of3-MCPD-esters is based on3-MCPD, that is,100%of3-MCPD-esters change into3-MCPD. So when we studythe toxicology for3-MCPD-ester, we get certain significance on the research oftoxicological data of3-MCPD.The result of Ames test shows that, three different doses of3-MCPD to thethree strains of TA97, TA102were negative, whether rat liver homogenate S-9 activation system. But for TA100strains, while dose is of5000μg/glass, whetherrat liver homogenate S-9activation system, the results are all positive; At the doseof1000μ g/glass for TA100strains and at the dose of5000μg/glass,1000μg/glass,200μg/glass for TA98strains, in no rat liver homogenate S-9activationsystem, the result is negative, in rat liver homogenate S-9activation system, theresult is positive. Bone marrow micronucleus test show that it is negative for3-MCPD on them. Sperm abnormality test results show that it is negative for3-MCPD on them when the dose is low and middle, and it has no teratogeny action.When the dose is high, it is positive for3-MCPD on them,and it has teratogenyaction.The results of MTT test is that, different concentration of3-MCPD can inhibitthe proliferation of293Ad5+cell and L02cell. And the activity are relativelyweak. In the cells of293Ad5+and L02, the cell activity of3-MCPD are23.6%and56.7%at the5mg/ml concentration. The inhibition of high levels of3-MCPD islarger to cells. And the toxicity in293Ad5+cells is higher than in L02cell. Whenthe concentration of RBPC are0.25mg/ml,0.5mg/ml and the concentration of GOare0.15625mg/ml,0.3125mg/ml,0.625mg/ml,1.25mg/ml for293Ad5+cell, andthe concentration of GS are0.0625mg/ml,0.25mg/ml for L02cell, the cells’activity could have certain improvement. And the other dose group of cells’activities would reduce, which shows that high levels of RBPC, GO, GS all havecertain toxicity to293Ad5+cells and L02cell.On the basis of toxicology experiment, We discussed the oxidative damagecaused by3-MCPD. The result is that, the3-MCPD can make the content ofT-AOC, GSH-PX, T-SOD and CAT decreased, and can make the content of MDAincreased. That illustrates that3-MCPD can cause oxidative damage of organism.