The Synthesis And Research Of Probes Detecting Palladium And PH Based On Aminocyanine

Recently, fluorescence imaging has emerged as one of the most powerful techniques to monitor targets and biological processes in the context of a living system To date, a large volume of fluorescent probes for a wide variety of targets have been developed. However, most of them have the absorption and emission in the ultra violet-visible light range, which renders them difficult to be employed for sensing and imaging targets of interest in living animals. By contrast, fluorescent probes with absorption and emission in the near infrared region are favorable for in vivo bio imaging because of minimum photo-damage to biological samples, deep tissue penetration, and minimum interference from background auto-fluorescence by bio-molecules in the living systems. Peng et al. reported a series of amine-substituted tricarbocyanines (aminocyanines) with large stokes shifts (>140nm) owning to intramolecular charge transfer (ICT) for the first time in2005. The interesting properties of the novel tricarbocyanines make them suitable for use as the scaffold for functional fluorescent probes.We design and synthesis the probe CyNl based on the scaffold of aminocynines and the reaction mechanism of Tsuji-Trost. We can get the obvious visualization change of color (from green to blue) and the ratio fluorescent signal through the reaction between the probe CyN1and palladium. The reaction condition is mild and good selectivity. The detection limit of CyN1is0.4ppb under the optimized condition, which is highly sensitivity. In particular, the probe CyN1is the first implementation of palladium ion in vivo fluorescence imaging due to the use of the biological compatibility of PEG400as reductant in the detection system.Based on the sturcture of aminocyanine leuco, we synthesis a case of pH fluorescent probe named CyH. Both of them have the large Stokes shift, whick can reach80-90nm, whether the probe CyH itself or its reaction product with H+. It has a high sensitivity with the fast reversible recognition ability of H+and the fluorescence intensity changes obviously within the scope of pH2.5-4.0. The interval of two fluorescent peak is almost150nm and the method of ratio detection can effectively avoid errors from the instrument and test conditions. CyH is no toxicity to cells, which can be carried out within the cell fluorescence imaging research and monitor the change of intracellular pH. What’s more, the probe CyH has good localization of lysosome that’s the organelle within cells.