Study On Silver Nanoparticles Enhanced Chemiluminescence And The Analytical Application

Chemiluminescence （CL） has become a focus in the area ofanalytical chemistry. Due to its advantages including high sensitivity,rapidity, wide linear range and simpler instrumentation. As a solutiontreatment method in non-equilibrium state, the flow injection （FI）could be coupled with CL, overcoming the poor reproducibility of CLand making the assays automatically. The main advantages ofcapillary electrophoresis （CE） are low reagent, sample consumption,high separation efficiency and short analysis times. When CL coupledwith CE, it has the advantages of high sensitivity, selectivity and canbe directly used for the separation and determination of tracecomponents in complex samples. FI–CL and CE–CL technologies havebeen applied to areas of environmental, biological, medical and so on.The main contents of this thesis as follows:（1）The method was based on the CL emitting reaction betweenpotassium ferricyanide and nitrazepam in alkaline medium withrhodamine6G as the CL enhancer. Ag nanoparticles （NPs） could further enhance the signal of the above CL system. On the basis, aFI-CL method was developed for the determination of nitrazepam. Thepossible mechanism of the systems was also explored by theUV―spectrum and CL spectrum. In the optimum conditions, the linearrange was1.0×10^-9–1.0×10-5mol/L and the detection limit was1.0×10^-10 mol/L. The recommended method has been successfullyapplied to the determination of nitrazepam in coca cola beverage,serum and urine.（2）Based on the enhancing CL of luminol–potassium ferricyanidesystem by AgNPs, a CE-CL method was established for thedetermination of L–tyrosine. Under the optimum conditions, the CLintensity was proportional to the L–tyrosine concentration in therange of5.0×10^-9–1.0×10-6mol/L, with the detection limit of1.2×10^-9 mol/L. The method has been successfully applied to the determinationof L–tyrosine in milk, serum and urine.（3） It has been found that the nicotinamide can inhibit thechemiluminescence of the luminol–potassium ferricyanide system.Based on this inhibition function, a capillary electrophoresis coupledwith chemiluminescence detection methed has been established forthe determination of the nicotinamide. Under the optimum conditions,the linear range for the determination of the nicotinamide was1.0x10^-8–1.0x10^-5mol/L, the detection limit was3.0×10^-9 mol/L. The method was applied to the determination of nicotinamide in differentmanufacturers in yogurt with the recovery rate from97%to103%.