The Isolation, Identification And Application Of Moderately Halophilic Bacterium In Environmental Modification

Moderately halophilic bacteria is the microbial population that is dependenting from0.5to2.5mol/L salt concentration. Moderately halophilic bacteria have a strong ability to adapt to the environment, simple nutritional requirements, widely distributed in nature and special physicochemical properties, which makes a wide range of applications in various fields, such as the fields of petroleum, chemical, pharmaceutical and food. Wastewater derived from petroleum, chemical, pharmaceutical and fertilizer industry has characteristics of high salt, high ammonia. Ammonia nitrogen wastewater will cause a lot of pollution. Physical or chemical treatment of high-salt wastewater has shortcomings of a high cost, low efficiency, and may cause secondary pollution. Traditional biological method has a great advantage when dealing with low salinity wastewater. But when wastewater salt concentration is too high, the microorganisms will lose the ability to degrade. Growth and metabolism of general functional microorganism will be inhibited in the inverse environment for example high salt, dry, high pH, high temperature, low temperature. Strains lost functions in different degrees. Maintaining or improving the functional microbial growth and metabolic capacity under high salt environment, including microflora resilience conferring, has important significance.In this paper, a moderately halophilic bacteria that degrades high-salt high ammonia nitrogen wastewater is isolated from a saltworks in Dalian mud filter. This strain was identified as Halomonas genus by16S rDNA. This strain was named Halomonas sp.BOl. This paper studies that the Halomonas sp.B01synthesis ectoine, reaching maximum at48h for1027.27mg/L. Ectoine gave bdellovibrio salt tolerance in certain salinity conditions. This paper studies the impact of different carbon sources, carbon-nitrogen ratio of its denitrification behavior, the best carbon source is sodium succinate, the best C/N is10; optimized pH of the strain on the wastewater ammonia nitrogen degradation, optimum pH is8; Halomonas sp.B01degrades high salt concentration ammonia nitrogen wastewater, concentration of ammonia nitrogen from1026.95mg/L reduced to19.99mg/L, the final conversion was98.05%.