Binding Peptide Screening Of Canine Distemper Virus (CDV) H Protein

Canine distemper is caused by canine distemper virus infection of acute, highlycontagious disease. Mortality rate of up to80%. Infected with a broad spectrum, andhost range is in the expanding. China’s wild animal protection, animal breeding anddog industry are causing serious harm. This study was to find a canine distemper virusreceptor inhibitory peptide to provide a basis for the treatment of canine distempervirus peptide drugs.In this study, suspected of having canine distemper disease compoundsuccessfully isolated a canine distemper virus, named ZA10-JL strain. Its H genesequences in the results shows the existence of multiple N-linked glycosylation sites,and a high predictive value of the glycosylation sites in the309-411amino acidresidue position and its unique wild strainthe phylogenetic tree was constructed basedon the H gene is further evidence of the separation of the Asia-I wild type strain. CDVH protein is located in the surface of the virus envelope is the first step in virusinfection of host cells and CDV infection of the host is necessary. Therefore, thisstudy is further designed and synthesized primers with restriction enzyme digestionsites, prokaryotic expression plasmid, expressing the H protein, the result was highlyexpressed in the functional areas of the head of the H protein expression in the formof inclusion body protein denaturation renaturation and purifiedto obtain high-purityH protein. Separation and Purification of the H protein substrate as a solid-phasescreening, the use of phage display random peptide library screening CDV H proteinin cell receptor binding site, the two may be related to the CDV H protein receptorbinding site sequence highlysimilar peptides. Peptide sequence SPRPMMRRIRKQand VSIEQESPKNVL.Two phage display peptides with the H protein with high affinity by phagedisplay peptide library screening technology.