Effects Of Xylooligosaccharides On The Immune Function And Intestinal Health Of AA Broilers

This research was designed to study the effects of xylooligosaccharides on the immune functions of AA broilers under nomal condition and Clostridium perfringens infection.In experiment1,5121-day-old A A broilers were randomly allocated into4groups to study the effects of xylo-oligosaccharides(XOS) on performance, serum indexes and cecal E.coli count of broilers. The diet treatments were:basal diet (as control), basal diet with100mg/kg XOS,150mg/kg XOS and200mg/kg XOS. The results showed that XOS supplementation in diet had no significant effect on the weight gain and the feed conversion rate for broilers (P>0.05). Diet supplemented with150mg/kg XOS decreased serum concentration of GLU in the starter phase and the concentration of TP, ALB and TC in the finisher phase significantly (P<0.05) XOS decreased the E.coli count in cecal, but not significantly.The second study was designed to evaluate the application of XOS as a feed additive in the diet of broilers, research for more potential immunity functions of XOS.6401-day-old AA broilers are randomly assigned to5treatment groups consisting of8replicates of16broilers. The diet of each group is as follow:(1) the control diet;(2) the control diet+80mg/kg XOS;(3) the control diet+120mg/kg XOS;(4) the control diet+160mg/kg XOS;(5) the control diet+200mg/kg XOS. Evaluate the effect of Xylooligosaccharides on performance and immunity of broilers through measuring the weight gain, serum IgA, IgG, SOD, MDA and apoptosis of immune organ. The results showed that:in the starter phase, diet with XOS had no effect on the performance, but improved the concentration of IgA, IgG and SOD in serum(P>0.05). XOS reduced the apoptosis rate of the thymus cells and intestinal mucosa cells. In the all period, diet with XOS reduced the weight gain and improved the feed conversion ratio, but not significantly. It had no significantly effect on the concentration of IgA, IgG, SOD and MDA in serum and reduced the apoptosis rate of the thymus cells and spleen cells(P>0.05).The third experiment studied the effect of XOS on the organ indexes, serum indexes, antioxidation and intestinal injured of broilers after Clostridium perfringens infection compared to the antibiotic.3841-day-old broilers were randomly divided into four groups. The diets of every treatment were as follows:the control group with basic diet); infection group(Clostridium perfringens infetion) with basal diet, antibiotic group(Clostridium perfringens infection) with the basal diet added40mg/kg bacitracin zinc and XOS group(Clostridium perfringens infection) with the basal diet added200mg/kg XOS. The results showed:the index of bursa of Fabricius was significantly lower after Clostridium perfringens infection but the index of thymus was opposite. Compared to the infection group, the index of bursa of Fabricius in the antibiotic group and the XOS group were higher and the index of thymus was lower respectively.2. The concentration of MDA in serum of broilers in the infection group was higher than other groups significantly and was lowest in the XOS group. Compared to the control group, the concentrations of TP、ALB and GLB in serum of broilers in other groups had on significant effects, but the concentration of UN in serum of broilers in the antibiotic group was higher significantly. The concentrations of TNOS and iNOS in serum of broilers after Clostridium perfringens were increased by12.96%(P<0.01) and6.95%(P<0.01) respectively. Compared to the infection group, the concentrations of TNOS and iNOS in serum of broilers in the antibiotic groups reduced by8.23%(P<0.05) and6.19%(P>0.05) while in the XOS group reduced by14.86%(P<0.01) and58.40%(P<0.01) respectively.