Association Study Between Polymorphisms Of TLR3and TLR9and Graves’ Disease In South Chinese Han Population

Graves’ disease (GD) is a common autoimmune thyroid disease (AITD) that issupposed to be caused by the interaction of both environmental agents and geneticfactors. It was reported that polymorphisms of members of Toll like receptor (TLR)family were associated with susceptibility to GD. In order to investigate thesusceptibility of TLR family to grave’s disease (GD) in South Chinese, a case-controlstudy was conducted in the present research. Four tagged SNPs from TLR3and TLR9were selected in present study, i.e. rs3775291and rs3775296of TLR3and rs187084and rs352139of TLR9. In total,377controls and355GD patients were genotyped bypolymerase chain reaction-sequence specific primers (PCR-SSP). The data showedthat female accounted for a larger proportion in patients with a ratio of~2.7to male.For TLR3gene, genotype frequency of rs3775296in female patients was significantdifference to that of control (p=0.04), but the frequency was disequilibrium inHardy-Weinberg equilibrium (p=0.03) in the female patients. Allele frequenciesanalysis showed no significant difference. Haplotypes of the two SNPs of TLR3werealso constructed and analyzed, but no significant difference was found. For TLR9gene, the allele frequencies of all subgroups except for that of rs352139of the femalepatients (p=0.04) were in Hardy-Weinberg equilibrium. Genotype, allele, andhaplotype analysis showed no significant difference between case and control. Inconclusion, our data indicates that there might be no significant association betweenTLR3or TLR9polymorphisms and GD susceptibility.