Effects Of Dexmedetomidine In Hyperalgesia And Calcitonin Gene-related Peptide Of Neuropathic Painful Rats

The Effects Of Dexmedetomidine On Behavior InNeuropathic Pain RatsObjective To investigate the effects of dexmedetomidine on behavior in neuropathicpain rats.Methods Thirty-two adult male SD rats were randomly divided intosham-operation group,chronic constriction injury(CCI)group,25ug/kgdexmedetomidine group,50ug/kg dexmedetomidine group,gave subcutaneousinjection dexmedetomidine or NS every day from postoperative to derivedpoint,determined mechanical withdrawal threshold(MWT) and thermal withdrawallatency(TWL) in preoperative1d,postoperative1d、3d、7d、14d. Results Comparedwith sham-operation group,the MWT and TWL of CCI group have significantlydecrease in postoperative1d、3d、7d、14d（p<0.05）. Compared with CCI group,theMWT and TWL of dexmedetomidine group have significantly elevate inpostoperative1d、3d、7d、14d（p<0.05）,and high-dose group have significantly elevatethan low-dose group. Conclusions dexmedetomidine can inhibit neuropathic paininduced by CCI,and high-dose have more effective than low-dose.the effects of dexmedetomidine on CGRPimmunocompetenceof spinal cord in neuropathic pain ratsObjective To investigate the effects of dexmedetomidine on CGRPimmunocompetence of spinal cord in neuropathic pain rats.Methods Thirty-two adult male SD rats were randomly divided into sham-operationgroup,chronic constriction injury (CCI) group,25ug/kg dexmedetomidine group,50ug/kg dexmedetomidine group,gave subcutaneous injection dexmedetomidine orNS every day from postoperative to derived point,take lumber spinal cord to doimmunohistochemistry after determination of behavior. Results Compared with sham-operation group,the CGRP immunocompetence ofCCI group have significantly elevate（p<0.05）. Compared with CCI group, the CGRPimmunocompetence of dexmedetomidine group have significantly decrease（p<0.05）,and high-dose group have significantly decrease than low-dose group.Conclusions dexmedetomidine can inhibit CGRP immunocompetence of spinal cordinduced by CCI,and high-dose have more effective than low-dose.Real Time PCR detected the expression level ofCGRP-mRNA in the spinal cord in neuropathic pain ratsObjective To investigate the effects of dexmedetomidine on expression level ofCGRP-mRNA in the spinal cord in neuropathic pain rats.Methods Thirty-two adultmale SD rats were randomly divided into sham-operation group,chronic constrictioninjury (CCI) group,25ug/kg dexmedetomidine group,50ug/kg dexmedetomidinegroup,gave subcutaneous injection dexmedetomidine or NS every day frompostoperative to derived point,take lumber spinal cord to do Real Time PCR afterdetermination of behavior. Results Compared with sham-operation group,theexpression level of CGRP-mRNA of CCI group have significantly elevate（p<0.05）.Compared with CCI group, the expression level of CGRP-mRNA ofdexmedetomidine group have significantly decrease（p<0.05）,and high-dose grouphave significantly decrease than low-dose group. Conclusions dexmedetomidine caninhibit the expression level of CGRP-mRNA in the spinal cord induced byCCI,and high-dose have more effective than low-dose.