Development And Preliminary Application Of ELISAs For Detecting Serum IgG Antibodies To HCoV-NL63and HCoV-229E

ObjectiveTo establish a serum enzyme-linked immunosorbent assay and to detect antibodies tothe human coronavirus NL63(HCoV-NL63) and human coronavirus229E (HCoV-229E)from serum samples of children in Fuzhou.MethodsRecombinant protein enzyme linked immunosorbent assay (ELISA) based on portionsof the nucleocapsid protein of HCoV-NL63and HCoV-229E were developedrespectively and were used to screen serum from442children and young adults.Results1、 In442serum specimens,277were positive for HCoV-NL63(62.7%). Thepercentages of seropositive individuals were50.6%（40/79） for infants≤6months old;67.1%(19/28)for infants>6～12months old;73.1%（125/171） for individuals>1～5years old;62.5%（55/88）for individuals>5～10years old; and50%（38/76）forindividuals>10～20years old.2、In442serum specimens,241were positive for HCoV-229E (54.5%). The percentagesof seropositive individuals were49.4%（39/79）for infants≤6months old;64.3%（18/28） for infants>6～12months old;70.8%（121/171）for individuals>1～5 individuals>10～20years old.Conclusions1、The methods of ELISA for detecting antibodies to HCoV-NL63and HCoV-229E weredeveloped successfully.2、Respiratory tract infection with HCoV-NL63and HCoV-229E is common in childrenin Fuzhou area though the prevalence usually vary among different age groups.3、The results of this research provides important information to prevention andtreatment of respiratory tract infection. The established methods has applicationpreospect in relative clinical analysis and researches.