TVPV1in The Nucleus Accumbens On Morphine Dependence And The Central Body Mass Regulation

The nucleus accumbens has become a hot nuclei to study the mechanismsof drug addiction, because it played a role of the hub and transit point in theformation of drug addiction. Which molecules regulate physiological andpathological functions in the nucleus accumbens is an important part of themajority of scholars. In recent years, studies have shown that TRPV1receptorsare widely distributed in the central nervous system, and it is particularly rich inexpression in the nucleus accumbens. Activation of TRPV1protein, the maincause of Ca²⁺ and other cations flow, regulate physiological functions orpathological mechanism of the occurrence of the form of increased intracellularCa²⁺ concentration, resulting in increased cell excitability where. Review of theliterature, TRPV1function of the nucleus accumbens is little studied. Recentstudies have shown that TRPV1can control body weight, and the study showedthat there have the functional links between the nucleus accumbens and the foodcraving and neuropathy morbidly obese,.These provides a guideline for researchTRPV1function of the nucleus accumbens. We explore the function of TRPV1 distribution of the central nervous system, especially distribution in the nucleusaccumbens, and whether it can affect the function of the nucleus accumbens andthus affect drug addiction, food craving behavior,.In this study, the TRPV1antagonist CPZ injection at different sites, including the nucleus accumbens andthe central nervous system, with the morphine-induced conditioned placepreference test and the rat body mass and fat accumulation measurements of thetwo experimental program to explore part of the physiological function of theTRPV1in nucleus accumbens. These provide scientific and theoretical basis forthe clinical treatment of drug addiction and neuropathy morbidly obese.Methods:1. Different doses of CPZ in the CPZ to the injection of morphinewithdrawal1W and3W nucleus accumbens, bilateral and unilateral nucleusaccumbens, bilateral dorsal striatum, bilateral the volts nuclear unilateraldeviation from the target at different injection sites at different dosesrats CPPbehavior tests scores.2. Observed and recorded to measure the activity of rats.3. Different doses of CPZ in the morphine withdrawal1W was injected intothe bilateral nucleus accumbens, bilateral dorsal striatum, bilateral nucleusaccumbens deviation from the target, record changes in rats body mass beforethe injection, after injection of short-term （1W） and after injection of long-term（3W）.4. Record is complete anatomy of the rats in each group observed thedifferences of body fat, in order to understand the fat accumulated in eachtreatment group compared with the control group.Results:1. TRPV1antagonist CPZ role in the bilateral nucleus accumbens can successfully dissipated rat mCPP dose and schedule to maintain the regressionand CPZ in a dose-dependent, the higher the amount of CPZ can longermaintain mCPP dissipated state.2. CPZ can not affect the activity of the rats, suggesting that the activity ofmorphine-induced conditioned place preference does not constitute interference.3. The role of the bilateral nucleus accumbens CPZ can effectively inhibitthe growth of body weight in rats, and inhibited the time course and dose levelof existence dependent, higher doses of CPZ can play a longer inhibitory effect.4.Only the the CPZ role of higher doses significantly inhibited the bilateralnucleus accumbens would fat accumulation.Conclusion:1. Inhibition of TRPV1in the nucleus accumbens could effectively inhibitthe role of morphine addiction, the performance of mCPP reduction of TRPV1inhibition may reduce the excitability of the nucleus accumbens is the impact onthe nucleus accumbens play an important molecular function of its drug craving.2. Inhibition of TRPV1in the nucleus accumbens can effectively inhibit thegrowth of rat body weight of TRPV1on body mass and fat accumulation maybe caused by TRPV1distributed in the nucleus accumbens to change theexcitability of the nucleus accumbens.