Protective Effect Of L-Carnitine On The Rat Retinal Nuron Cells In High-glucose

OBJECTIVE:Building a primary culture of neonatal rat retinal neural cells in vitro cellular, then establish the model of the retinal neural cells induced by high glucose after giving L-Carnitine, to investigate the effect of L-carnitine on the rat retinal nuron cells in high-glucose.METHOD:Cell suspension with different sizes of cell aggregates was made from postnatal1-3days Sprague-Daw rats by using0.25%trypsin digestion, and then it was planted to culture flasks covered with Poly-L-lysine. The cell growth was observed by phase contrast microscope, and the cells (7-9days) were identified by immunofluorescence by using neuron-specific enolase (NSE). The experiment used cells(8-10days), and was divided into five groups:normal cell culture group (control group), high glucose cellular model group, high sugar cellular model group+L-carnitine (50μmol/L), high sugar cellular model group+L-carnitine (100μmol/L), high sugar cellular model group+L-camitine (200μmol/L). Cell viability was determined by MTT, oxidative was displayed by using electron spin resonances (ESR) to dissect the release of nitrogen monoxide (NO) and reactive oxygen species (ROS).RESULT:Most retinal neural cells were NSE-positive detected by immunofluorescence. Cell viability decreased obviously in high-glucose (P<0.05), and the best protective effect of L-carnitine was that drug concentration was100μmol/L, and took action after48h. It took cytotoxic reaction in200μmol/L. NO and ROS released increasedly in high-glucose by ESR (P<0.01), and those can be inhibited by L-carnitine.CONCLUSION:NO and ROS released increasedly in high-glucose, and oxidative can be inhibited by L-carnitine.