Experimental Study The Prestin Distribution At The Membrane Of Single Outer Hair Cells Of Cochlea In Newborn Rat

Objective:To investigate the isolation and culture and the Prestin Distribution at the Membrane of Single Outer Hair Cells of Cochlea in Newborn Rat Methods In this experiment.Methods:single cell of Outer Hair Cells was geted from9-day-old wistar rat, the distribution of prestin was examined by immunofluorescent staining and fluorescence microscopy.Results:The staining was expressed on the lateral membrane; the base membrane was also expressed; on the bottom side of the membrane and the lateral membrane-binding site, The staining was not expressed.Conclusion:OHC is separated and cultured for24hours. Prestin is mainly Distributed on the lateral membrane, and Basal membrane is also a small amount of expression. The density of Prestin is the highest on the lateral membrane.