| Objective To study the effects of the recombinant lentiviral vector for RNA interference(RNAi) of Cdx2gene (pLL-Cdx2-siRNA) on the drug resistance of human gastric cancer resistance cell line SGC-7901/DDP.Methods Cdx2-siRNA(experimental group) and pLL-3.7(vector group) were transfected into gastric cancer resistance cell line SGC-7901/DDP. After72hours, the expression of protein and Cdx2mRNA in resistance gastric cancer cell line SGC-7901/DDP were detected by western blotting and Real-time quantitative PCR methods respectively; MTT assay is used to investgate the effect of DDP, ADR and5-FU on the cell growth of human gastric cancer resistance cell line SGC-7901/DDP; Flow cytometry assay is used to investgate the effoct of Cdx2-siRNA on ADR accumulation of gastric cancer resistance cell line SGC-7901/DDP; The cell cycle arrest and apoptosis rate were detected by flow cytometry assay.Results After the experimental group and vector group were transfected successfully, the expression of protein in resistance gastric cancer cell line SGC-7901/DDP was obviously inhibited, about69.4percent and72.51percent lower than the vector group and control group(P<0.05); the expression of mRNA in resistance gastric cancer cell line SGC-7901/DDP was obviously inhibited, about75.4percent and79.32percent lower than the vector group and control group(P<0.05); the drug sensitivity of cell line SGC-7901/DDP was obviously increased about DDP, ADR and5-FU(P<0.05); Cdx2-siRNA have increased the ADR accumulation of gastric cancer resistance cell line SGC-7901/DDP (P<0.05); more mean DNA content of SGC-7901/DDP cells accumulated at G2/M phase, the mean DNA content at G2/M phase of experiment group was (11.93±1.52)%, the difference have atatistical significance compered with vector groups (0.26±0.02)%and control group (0.35±0.02)%(P<0.05); After transfection, the apoptosis rate of SGC-7901/DDP cells was (31.13±1.28)%, the difference have atatistical significance compered with vector groups (16.58±1.50)%and control group (13.18±1.64)%(P<0.05).Conclusions The expression of protion and mRNA were obviously inhibited by Cdx2-siRNA, and the drug sensitivity of SGC-7901/DDP cells about DDP, ADR and5-FU was incresaed, increase the accumulation of ADR, and the Cdx2-siRNA made the mean DNA content of GO/G1down and accumulate at G2/M phase. |
PDF Full Text Request