Research On The Correlation Between Fitc-csnrdarrc Molecular Probes Targeting Tags And Bladder Transitional Cell Carcinoma Grading

ObjectiveThe peptide CSNRDARRC is to demonstrate the bladder transitional cell carcinoma specific peptides obtained by technological screening of peptide library through phage. This subject uses laser scanning confocal microscope to quantitative detect the combination status of FITC-CSNRDARRC molecular probes with bladder transitional cell carcinoma targeting of different pathological grades, explores the correlation between FITC-CSNRDARRC molecular probes targeting tags with pathological grading of bladder transitional cell carcinoma, and provides a new method for early diagnosis and grading diagnosis of bladder cancer. This molecular probe is further used in vivo studies and clinical application, providing certain experimental basis and foundation.Methods1. Compose FITC-CSNRDARRC molecular probes.2. According to the pathological grade group the organization of the bladder transitional cell carcinoma, prepare sliced specimens of bladder transitional cell carcinoma tissues, and FITC-CSNRDARRC molecular probes targeting tags.3. To make laser scanning confocal microscope quantitative detection of the prepared specimens, and obtain data and fluorescence images.ResultsFluorescent expression can be seen in grade Ⅰ, Ⅱ and Ⅲ bladder transitional cell carcinoma tissues, and the fluorescence value is positively correlated with tumor grade (rs=0.942,P＜0.01); and the difference of the mean fluorescence value among these three groups has a statistical significance (P＜0.017), and then make comparison between two groups, the difference between grade Ⅰ bladder carcinoma group and grade Ⅱ bladder carcinoma group has a statistical significance (P＜0.05), the difference between grade Ⅰ bladder carcinoma group and grade Ⅲ bladder carcinoma group has a statistical significance (P＜0.05), and the difference between grade Ⅱ bladder carcinoma group and grade Ⅲ bladder carcinoma group has a statistical significance (P＜0.05). ConclusionThe FITC-CSNRDARRC molecular probes targeting tags is positively correlated with bladder transitional cell carcinoma pathological grading, which is able to distinguish bladder carcinoma of different pathological grades.