Inhibitory Effect Of Xiaoaiping Injection Combined With Octreotide Acetate On Mouse Transplanted Liver Cancer

Objective:Hepatocellular carcinoma (HCC) is a common malignancy tumor, and its mo rbidity and mortality in our malignant tumors accounted for the front position, which is a serious threat to people’s health and life. It is still lack of efficacy, fewer side effect s of treatment of HCC drugs in clinical. Chinese medicine Xiaoaiping fluid (XAP) is a commonly used anti-cancer drugs in recent years, there are a large number of experim ents to prove its good therapeutic effect in HCC; Octreotide (OCT) play the role of ant i-hepatoma cells by somatostatin receptor (SSTR), some studies have shown that better i n recent years; But there is very few reports on the the XAP combined with OCT treat ment of HCC. Therefore, experiments want to explore the different concentrations the X AP combined with OCT in vivo antitumor effect on HCC and its possible mechanism o f action through the establishment of H22hepatoma xenograft mouse model.Methods:After the establishment of mouse subcutaneous tumor model the experimental animals were randomly divided into normal group, model group, XAP low, medium an d high dose group(10g/kg·d,20g/kg·d,40g/kg·d), OCT group(100μg/kg), and the group of XAP low, medium and high dose groups were combined with OCT, every group has10mice; and begins to a daily intraperitoneal injection. To measure every two days th e length and width of transplanted tumor, then to calculate the tumor’s volume and dra w the tumor growth curve; After two week’s treatment, all the animals were sacrificed t hrough bloodletting of the eyeball after24hours; the tumor and spleen tissue were stri ped, and then the tumor growth inhibitory rates and the spleen index of mice were calc ulated. The combination therapy efficiency gains were evaluated according to the value of q. Organization pathological changes observed by HE staining; and the level of cytok ine production of Thl(IFN-γ、IL-2) and Th2(IL-4、IL-10) were detected by ELISA met hod. The apoptosis of the tumor tissue was detected by TUNEL method and the expres sion of apoptosis protein Bax, Bcl-2were determined by immunohistochemistry(IHC).Results:1. Compared with that of control group, the volume and weight of implanted tumo rs in XAP medium and high dose group、and combination group were significantly sma Her and lighter(P<0.05), compared with that of control group, the volume and weight of implanted tumors in XAP low dose groups and OCT group were no difference(P>0.05); compared with that of any other group, the volume and weight of implanted tumors in combination group were significantly smaller and lighter(P<0.05).2. Tumor inhibition rate with the increase of the concentration of XAP increased, which was dose-dependent manner. Single-agent OCT also inhibited tumor. Single agent OCT group, the XAP low-dose group compared with the model group was no significa nt difference(P>0.05); the role of the combination group was significantly better than th e single drug group and the same concentration of octreotide monotherapy Xiaoaiping g roup. The q value of the XAP low, medium and high dose joint OCT were0.9197,1.1233,1.2826; That showed the XAP combined with OCT with a synergistic effect, the XAP high dose+OCT group was the best inhibitory effect on the tumor.3. Spleen index:Compared with control group, the spleen index of OCT group, X AP each dose group, the combined treatment group were a certain increase, the role of the combination group was significantly better than the single drug group and the sam e concentration of octreotide monotherapy Xiaoaiping group.4. HE staining showed:Tumor necrosis of the combination group was significantly more than the saline group, Compared with that of control group, the necrosis of the X AP each dose group and the OCT group were increased to varying degrees.5. In the model mice, serum IFN-gamma, IL-2levels decreased, IL-4, IL-10levels increased by showing a Th2-type immune response status. and the combined group in correcting the imbalance of cytokines was more effective than the drug alone group, O CT group compared with the model group, the difference was not statistically different (P>0.05).6. The AI of tumor issues in each test group is larger than that in control group, t he AI of tumor issues in combination group is more than the drug alone group (P<0.05). By the comparison of AI of tumor issues, there were no difference between OCT gr oup and control group. All treatment groups could cut tumor-bearing mice inhibited tum or cell apoptosis Bcl-2gene expression,to increase the expression of apoptosis promotin g gene Bax for the occurrence of apoptosis.and the activ ity of nature kill cell in splee n was rose, the combination group was best.Conclusion:XAP and OCT could inhibit the growth of H22hepatoma carcinoma transp lanted, the inhibitory effect of the combined group is superior to single the XAP group and the OCT group, the XAP combined with OCT has a synergistic effect, and XAP hi gh combined with OCT is the group with the best concentration of tumor-inhibition. The role of inhibition of tumor growth may pass to increase the apoptosis rate of H22tum or cells in vivo and also to improve cellular immunity of the mice bearing H22.