| Background：Chronic obstructive pulmonary disease (COPD) is a state characterisedby progressive airflow limitation that is not fully reversible. The specific pathogenesis ofthis disease is not clear. After the acute stage, although the clinical symptoms of COPDpatients eased, but their lung function is still declining and even continue to deteriorate.Due to their own defense and immune function decline as well as a variety of harmfulfactors the outside world, the disease of these patients is often recurrent. With theprogression of the disease, it may gradually produces a variety of cardiopulmonarycomplications. People have found many risk factors include smoking, respiratory infections,air pollution and other external factors and air hyperresponsiveness and other internal andexternal factors. The incidence of COPD may be related to pulmonary and systemicimmune disorder. Attention has traditionally been centered on the roles of macrophages andneutrophils in COPD, in recent years, the abnormal lymphocyte function have beenreceived more and more attention. T lymphocytes, B lymphocytes and natural killer (NK)cells have a key role in host defence against lung infectious pathogens. Cytotoxic Tlymphocytes (CTL) and natural killer (NK) cells are fundamental to host defence againstpathogenic microorganisms. They have cytotoxic functions mediated by release ofcytotoxic granules and also produce cytokines. T, B, and NK cells may play an importantrole in the development of COPD. Until today, the changes in the peripheral blood immunecells of patients with COPD do not reach a unanimous conclusion, some report that itincreases, some report that it reduces, some say there is no change, the results is lack ofconsistency. There are few research about immune function changing after the COPDpatients were treated.Objective: To investigate the peripheral blood T, B, and NK immune cells changingof COPD patients. Provide theoretical support for the immunomodulatory treatment ofpatients with COPD. Analyze the relationship between these cells and FEV1/FVC.Methods: The experiment consists of40participants,40patients were diagnosed withCOPD,20healthy people were the group of control. We study the following indicators: theperipheral blood T cell subsets (CD3+and CD4+and CD8+and CD4+/CD8+), B cell and natural killer cell NK, these cells are determined by flow cytometry.40COPD patientsare treated with anti-inflammatory, expansion of the airway and other conventionaltreatment for about2weeks, white blood neutrophil cell percentage decrease to normal andthe patients symptoms improved and the patient will discharge, we again extract thepatient’s peripheral blood samples to detect the number of T、B、and NK cells. We measurethe T，B and NK cell difference between the two groups. In addition, we analyze therelationship between lung function and cellular immunity.These indicators are compared todetermine whether there are statistical differences.Results:1,Compare to the control group, in the exacerbation of COPD,the CD3+, CD4+,CD4+/CD8+, NK and B cells were lower, there are significant with differences (P<0.05);CD8+T cell increased, but there was no significant statistical difference (P>0.05);2, Compared with the exacerbation of COPD, CD3+, CD4+,CD4+/CD8+, NK andB cells were increased in stable period of COPD, but there was no significant difference(P>0.05),3, Compared with healthy controls, CD3+, CD4+,CD4+/CD8+, NK and B cellswere significantly lower in stable period of COPD (P<0.05);4, Lung function(FEV1/FVC) have positive correlation with CD4+T cell、CD4+/CD8+and NK cell.Conclusion1, The immune of COPD patient was dysfunction, cellular and humoral immunedecline;2, when COPD patients discharged after treatment, the immune resistance improve;the immune resistance did not restored to normal levels immediately, so people shouldavoid re-infection at this time;3, The lung function FEV1/FVC have some relationship with these cells, and we needto study further. |
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