Study Of Electrospun Silk/Poly(ε-caprolactone) Tubular Material

Imitating the fiber orientation in different gradations of nature blood-vessel wall toprepare tubular scaffold with excellent properties which is used for conducting andregerating blood vessel in tissue engineering is a possible way to repair defective vessels.Electrospinning is one of the primary ways that can produce micro-and nano fibersscaffold for tissue engineering. SF/PCL tubular scaffold, whose fibers are oriented alongthe radial direction of roller, is with good cellular compatibility. It sets a techinalfoundation of material preparation for developing tubular scaffold with controlled fiberorientation used for tissue regeneration in future.First, the SF/PCL films were fabricated by electrospinning with the condition asfollows, SF/PCL were dissolved in hexafluoroisopropanol (HFIP). The concentration was6wt%, the voltage12kV, and the flow rate1ml/h. The collection plate was positioned at adistance of10cm from the needle tip. The ratio of SF/PCL were respectively100/0,70/30,50/50,30/70and0/100. In vitro cell culture showed that the adhesion, spread andproliferation of L929cells on the SF/PCL films were observed by LSCM, SEM and MTT.The results revealed that L929cells stretched on the SF/PCL films, and the cell numberincreased with the prolongation of the culturing time. The rate of cell adhesion andproliferation was higher when cultured on the SF/PCL films with the ratio of70/30and50/50.Second, SF/PCL films were collected by tablets and roller device, and their structureand physical properties were compared respectively. The results showed that theaggregation structure of SF/PCL films prepared by roller collecting and tablet collectinghad no significant difference. The fiber collected by roller device was with less averagediameter and lower break elongation, but the break strength was improved due to drafting. Third, Electrospun SF/PCL tubular scaffold was prepared by homemade device, andthe ratio of SF/PCL was50/50. The fiber extend of SF/PCL tubular scaffold was evaluatedby LabVIEW, at the same time, the influence of different speeds of roller collecting to fiberorientation in tubular scaffold was explored. The results demonstrated that when thediameter of tubular was3mm, the fibers had good arrangement along the radial directionof roller at the rotational speed of1500r/min or2000r/min. XRD and FT-IR showed thatrotational speed had no obvious effect on aggregation structure of SF/PCL tubular scaffold.The results of stretching test and Dry, Wet surgical suture strength showed that when theroller speed reached1500r/min or2000r/min, the radial strength and Dry, Wet surgicalsuture strength of SF/PCL tubular scaffold were both increased.