| ObjectiveTo investigate the gastroprotective effects of ghrelin against ethanol-induced acute gastric injury in rats and possible mechanism.MethodsPart 11.64 female Sprague-Dawley(SD) rats were divided into four groups and were intragastrically administrated as follows:Control group(2mg/kg 0.9%NaCl ip+lml/100g 0.9%NaCl ig);Ethanol model group(2ml/kg 0.9%NaCl ip+lml/100g 75%ethanol ig);Low-dose ghrelin group(2μg/kg ghrelin ip+lml/100g 75%ethanol ig);High-dose ghrelin group(20μg/kg ghrelin ip+lml/100g 75%ethanol ig).Rats were anaesthetized and the stomachs were removed after 2 hours.2. The ulcer index of gastric mucosal lesions in rats were calculated.3. Gastric mucosal inflammation were assessed by hematoxylin-eosin (H&E) staining.4. eNOS/iNOS and COX-1/COX-2 mRNA expression in these specimens were verified by semi-quantitative reverse transcriptional polymerase chain reaction (SqRT-PCR).Part 21.64 female SD rats were divided into four groups and were intragastrically administrated as follows:Control group(2mg/kg 0.9%NaCl ip+lml/100g 0.9%NaCl ig);Ethanol model group(2ml/kg 0.9%NaCl ip+lml/100g 75%ethanol ig);Ghrelin group(2μg/kg ghrelin ip+lml/100g 75%ethanol ig);Capsazepine group(20μg/kg ghrelin ip+lmg/kg Capsazepine ip+lml/100g 75%ethanol ig).Rats were anaesthetized and the stomachs were removed after 2 hours.2. The ulcer index of gastric mucosal lesions in rats were calculated.3. The inflammatory lesions of gastric mucosa were assessed by hematoxylin-eosin (H&E) staining.4. eNOS/iNOS, COX-1/COX-2 and CGRP mRNA expression in these specimens were verified by SqRT-PCR5.The levels of CGRP in these specimens were measured by Enzyme-linked immunosorbent assay (ELISA).Results Part 11. The ulcer index of ghrelin groups were significantly less than that of ethanol model group, and were positively correlated with dosages of ghrelin.2. Compared with ethanol model group,the gastric mucosal inflammation lesions of ghrelin group were significantly reduced, and were positively correlated with dosages of ghrelin.3. Compared with Ethanol model group,grhrein groups were characterized by no changes of COX-1 mRNA level, but iNOS mRNA and COX-2 mRNA decreased, eNOS mRNA increased in a dose-dependent manner.Part 21. The ulcerative index of capsazepine group were significantly greater than those of ghrelin group.2. Compared with ghrelin group,the gastric mucosal inflammation lesions of capsazepine group increased significantly.3. Compared with ghrelin group, there was no change of COX-1 mRNA level in capsazepine group,but mRNA levels of COX-2 and iNOS increased while eNOS and CGRP decreased.4. Compared with ghrelin group, the levels of CGRP in gastric tissue of capsazepine decreased.ConclusionsGhrelin had protective effects on ethanol-induced acute gastric mucosal injury in rats.The mechanism of the protective effects was related to CGRP which can be involved in regulating capsaicin-sensitive nerves and thus participated in the regulation of the activity of NOS and COX. |
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