Fibroblast Growth Factor 1 (FGF1) Different Molecular Networks And Prediction Of Mechanisms Function In Chimpanzee And Human Left Cerebrum

In this paper, our aim is to construct FGF1 activated and inhibited up-and down-stream network in chimpanzeeand and human left cerebrum separately. We used microarrays containing 12,558 genes from 15 chimpanzee and 14 human left cerebrum samples in GEO data set. We identified significant expressed genes markers using significant analysis of microarrays. We normalized data by log2 and analysed by cluster analysis method. First, we established total network of 441 significant high and low expression molecules （fold change≥2） from 12,558 genes by GRNInfer. Second, we identified different high-and. low-expression FGF1 network from our total constructed network in chimpanzee and human left cerebrum. Third, we identified FGF1 up- and down-stream network in chimpanzee and human left cerebrum. Fourth, we further identified activated and inhibited molecules from FGF1 up- and down-stream network in chimpanzee and human left cerebrum. Our methods for biological analysis have got a high evaluation by experts in the biomedical field and we have published several papers in international journals indexed by SCI, such as Cell Biochemistry and Biophysics （impact factor 4.311）, Journal of Cellular Biochemistry （impact factor 3.121）, Cell Proliferation （impact factor 2.742）, Cellular and Molecular Neurobiology （impact factor 2.423）.In this paper, we find that FGF1 is related to extracellular region, extracellular matrix （sensu Metazoa）, extracellular space, protein binding, growth factor activity, heparin binding; angiogenesis, induction of an organ, signal transduction, development, cell proliferation, fibroblast growth factor receptor signaling pathway, morphogenesis, cell differentiation, lung development, positive regulation of epithelial cell proliferation （GO database）. We constructed the low-expression fibroblast growth factor 1 （FGF1） DNA damage-mediated double-strand break combination repair coupling cell division to telomere maintenance for anti-apoptosis network and low-expression fibroblast growth factor 1 （FGF1） inhibited network of innate immunity-driven transcription coupling telomere maintenance via telomere shortening for regulation of apoptosis in chimpanzee left cerebrum compared with high-expression （fold change≥2） human left cerebrum. And we also constructed the high-expression fibroblast growth factor 1 （FGF1） defense response-mediated migration coupling signal with transport to oxidative metabolism for dendrite development network and high-expression fibroblast growth factor 1 （FGF1） inhibited network of migration coupling DNA repair to transcription for positive regulation o axon extension in human left cerebrum compared with low-expression (fold change≥2 chimpanzee left cerebrum. Our result showed that upstream C10ORF10, CDC25B, LOH11CR2A, RAD50, SAPS2, STAMBP activated FGF1; AL049278, AL080232, CFHR1, CTBP1, DDX3Y, RNF2, RPP14, SARM1, TERF1₁ inhibited FGF1 and the downstream FGF1- activated & FGF1-inhibited have no results in chimpanzee lef cerebrum. Our result also showed that upstream CTRL, GPD1, LGALS3BP, MAP1B₃, PCDHGA8, PCSK6, PDIA2 activated FGF1; DTNA, FOXN3₁, MAPT, NAIP, NR1D2₂, SLC25A46, SMG1 inhibited FGF1 and the downstream FGF1 activated MGC15523, NUPR1, UBXD2 and FGF1 inhibited ISCA1, PSMA4, U79289 in human left cerebrum. Our research for fibroblast growth factor-1 has an important role on neurodegenerative disease and clinical treatment, and is helpful for the use of genetic research for diseases of pathological significance.