Curcumin Affects The Expression Of Id1mRNA In Prostate Cancer PC3Cells And Lung Cancer H446Cells

Background:As the knowledge of tumorigenesis mechanism being more profound and extensive, scientists have focused on the correlation between malignant tumors and Idl (inhibitor of differentiation or inhibitor of DNA bindingl). It has been proved recently that over expression of Idl is related to the growth and malignancy in some types of tumor cells. Studies have demonstrated that Idl is over expression in many tumor cell lines. Furthermore, it has been confirmed that over expression of Idl occurs in many malignant tumors, including prostate cancer and lung cancer. Recently, curcumin has been a focus of the research on prevention and therapy of tumor. It is unclear if curcumin affects the expression of Idl in prostate cancer cells and lung cancer cells.Objective:To observe the effects of curcumin on the gene expression of Idl in androgen-independent prostate cancer PC3cells and lung cancer H446cells. To investigate the correlation between curcumin and cell viability, migration and apoptosis in PC3and H446cells, in order to find more effective measures for prostate cancer and lung cancer.Methods:The quantity of Idl mRNA was detected by real time quantitative reverse transcription-polymerase chain reaction (RT-PCR) in PC3and H446cells treated by curcumin. The cell viability of PC3and H446cells was evaluated by MMT assay. The cell migration was determined by scratch wound assay. The cell apoptosis was assayed by Hoechst staining.Results:In PC3and H446cells, the relative quantity of Id1mRNA reduced significantly by curcumin (p<0.01). The cell inhibition rate and cell apoptosis rate increased obviously by curcumin (p<0.01). The cell migrating distance was signifancant shorter by curcumin (P<0.05). The above changs of PC3and H446cells were correlated with the concentration of curcumin in a dose dependent manner (P<0.01).Conclusion:1. Curcumin can reduce the expression of Id1mRNA in a dose dependent manner in androgen-independent human prostate cancer PC3cells and small cell lung cancer H446cells.2. Curcumin can inhibit the cell viability, migration ability and induce cell apoptosis in a dose dependent manner in androgen-independent human prostate cancer PC3cells and small cell lung cancer H446cells.