The Construction Of Animal Model And Clinical Research On CT Quantitative Diagnosis In Fatty Liver

Objective:To study the correlation between the fat invasive degree and CT value on animal model,and the real applying significance of different quantitative MSCT diagnostic techniques in Human quantitative fatty liver diagnosis.Materials and methods:1The experimental research in quantitative CT evaluation of animal model with fatty liver.1.135healthy New Zealand white rabbits without gender difference were included and divided into two groups randomly:5normal rabbits were in the control group with routine feeding;30were included in the experimental group and feed with rich-in-fat diet,all were feed by12to20weeks.5,5,13from experimental group and1,1,3rabbits from control group were chose randomly and received CT examination in12th,16th,20th week respectively.1.2CT scan:The objects chosen from both groups as mentioned above were undergone total liver CT scan under muscle anesthesia with64rows of64-slice GE Light the Speed VCT at12th、16th、20th week respectively.1.3The processing and study of CT image:to transmit the scan data into the GE Advantage Window4.4workstations, and then measure the CT value of the liver as well as paraspinal muscle, meanwhile, calculate the CT value ratio of liver/erector spinae muscles on the obtained image.1.4Some biochemical indicators were test with samples coming from ear vein blood before the animal model’s CT scan.After that, liver tissue biopsy was done. To compare the histopathological results in animal model for fatty liver and CT value on images2The study based on clinical experiment in quantitative diagnosis of fatty liver.2.1Objects checking:a total number of200cases,covering normal people in outpatient clinician and fatty liver patiens from the First Affiliated Hospital of GuangXi Medical University during the period of December in2010to January in2012,and among them,31had biochemical results.2.2CT scan:Utilizing64rows of64layers of GE Light Speed to scan the liver routinely.2.3The processing and study of CT images:to transmit the scan data into the GE Advantage Window4.4workstations,and then measure the CT value of liver, spleen, inferior vena cava, abdominal aorta, erector spinae, and rectus abdominis, eventually all the CT value ratio such as liver/spleen, liver/inferior vena venous, liver/abdominal aortus, liver/erector spinae muscle, liver/rectus muscle. According to the the NAFLD CT diagnostic criteria developed by the Chinese Medical Association of Hepatology fatty liver, alcoholic liver disease study group in February2006[1] of all observations divided into normal, light, moderate and severe four groups.2.4All patients with fatty liver CT images of diagnostic radiology laboratory for three high qualification physician with the diagnosis the vascular relative density method. 3Research context:(1) To observe the change in the pathological classification and CT of different animal models for fatty liver(2) To analyze the relationship between the cholesterol content in animal models and varying invasive degree in the fatty liver.(3) To evaluate the quantitative diagnostic value in fatty liver in comparison of different technique.4All count indicator input SPSS16.0statistical package (overall mean number of t-test, ANOVA analysis, rank correlation analysis, etc.) and Microsoft Office Excel2003for statistical analysis.Result:1The experimental results of the CT quantitative study in fatty liver disease animal model1.1The pathological findings confirmed the indicators of the relevant variables and classification of animal models:The experimental group rabbit at different experimental stage deaths7, the remaining23experimental group and5normal group completed the experiment. Pathological findings in normal liver, mild, moderate and severe fatty liver5,8,8,7only; Liver CT values were70.72±6.64Hu,56.39±3.04Hu,47.65±3.10Hu,37.47±7.81Hu; CT value ratio of liver/erector spinae were1.03±0.14,0.88±0.06,0.72±0.11,0.59±0.11; serum total cholesterol2.03±0.64mmol/L, and18.19±5.27mmol/L, and5.29±4.69mmol/L, and33.38±4.68mmol/L1.2Experimental animals measured several variables indicators using analysis of variance (P<0.05) significant differences between groups, no difference between addition to the normal and mild CT value ratio of liver/erector spinae (P>0.05); Spearman rank analysis showed a significantly (P <0.05) with the degree of fatty liver. CT value of animal liver, liver/erector spinae CT value ratio of fat degree of a negative correlation; serum total cholesterol and fat were positively correlated; liver CT value of each drop10Hu, serum total cholesterol raised12.08mmol/L.2Results of clinical studies of fatty liver quantitative diagnosis2.1The CT scan observation results of Selected populations were divided into four groups:normal group,50cases;Mild, moderate and severe fatty liver group of50cases. Group of liver CT values were:61.82±4.10Hu,42.45±4.58Hu,34.30±3.82Hu,16.12±9.20Hu; liver/spleen CT value ratios were1.21±0.10,0.82±0.08,0.64±0.07,0.31±0.17; liver/inferior vena cava CT value ratios were1.60±0.19,1.06±0.15,0.86±0.13,0.42±0.25; liver/abdominal aortic CT value ratio were1.45±0.14,0.97±0.12,0.79±0.13,0.39±0.23; liver/erector spinae CT value ratio of1.09±0.12,0.79±0.08,0.63±0.08,0.31±0.18; liver/the rectus meat CT value ratio were1.19±0.14,0.85±0.12,0.66±0.09,0.32±0.20.2.231Cases of the relationship between serum cholesterol and liver CT value by linear regression analysis, derived liver CT value declining10Hu, serum total cholesterol was raised by2.10mmol/L2.3The diagnosis of fatty liver CT value of130cases, the accuracy rate of86.7%; vascular relative density of diagnosis of fatty liver142cases, the accuracy rate of94.7%;(x2=5.672, P=0.017<0.05) between the two are different. The six cases of pathologically confirmed fatty liver and the corresponding level of CT diagnosis.2.4Clinical cases measured several variables index by analysis of variance and rank sum test (P<0.05) significant differences between groups; Speannan rank analysis showed significant correlation (P<0.05) as to the degree of fatty liver. The CT values of Human liver, liver/spleen CT values, liver/CT value of the erector spinae muscle, liver/the rectus CT value, the liver/abdominal aortic CT values and liver/inferior vena cava CT value ratio of the correlation coefficient was-0.958,-0.949,-0.947,-0.939,-0.933,-0.931.Conclusion:(1) CT values could reflect fat content in the fatty liver of animal models indirectly; when the CT value in animal’s liver decreased by16.5Hu, it means the hepatic fat content increased by10%correspondently.(2) Liver/spleen CT value ratio is the best quantitative diagnostic technique in Human fatty liver,and it is negative correlative to fat invasive degree and the correlation coefficient is-0.958.