The Influence Of Glargine Insulin, Detemir Insulin And Human Insulin In3T3-L1Adipocytes On The Expression Of PPAR γ2mRNA

Objective:The aim of this investigation is to exam the the expression of PPARy2mRNA under the effect of glargine insulin, detemir insulin and human insulin in3T3-L1adipocytes and possible mechanisms.Methods:1. Culturing3T3-L1preadipocytes in vitro is to differentiate it into mature adipocytes. There were4major sample groups based on the different types of induction medium containing insulin. They are glargine insulin (powder), glargine insulin (injection), detemir insulin(injection) and human insulin (powder). Next step, each major group was divided into3small groups based on the insulin concentrations as100nmol/L,500nmol/L and1000nmol/L respectively. Besides, a control group containing10nmol/L human insulin was set up. All groups of mature adipocytes were culrured for10days. The sample size of each group was3, which was repeated for3times.2. Withdrawing overall RNA from cells is to make reverse transcription as cDNA. Using RT-PCRmethod to tset the relative expression level of PPARy2mRNA.3. Evaluating the experienment data, which adopting SPSS13.0statistic software, is to testify its statistical significance. All values were presented as average±standard deviation(x±s). The comparison among several quantitative determine group was using one-way analysis of variance(ANOVA). The comparison between two groups was adopting LSD-t test. The examination standard was set as a=0.05. When P<0.05, the values had statistically significance.Results:1.The comparison among the different insulin affecting3T3-L1adipocytes on the expression of PPAR γ2mRNA:Rising the concentration of glargine insulin(powder), glargine insulin (injection) and human insulin(powder), each group of PPAR γ2mRNA expression increased gradually. It indicated the promotion effect of1000nmol/L insulin was the stronger than that of500nmol/L, and that of100nmol/L was the weakest. All above value were statistically significant.2. The comparion among the different insulin in the same concentration on the expression of PPAR Y2mRNA:under the induction of the100nmol/L,500nmol/L and1000nmol/L, it was found that the promotion effect of human insulin(powder) was stronger than that of glagine insulin(inj ection), and that of detemir insulin was the weakest. Total difference has statistically significance(P<0.05). There was no obvious difference between the glargine insulin(powder) group and the glargine insulin(inj ection) group.Conclusion:1.During the induction differentiation from glargine insulin, detemir insulin and human insulin to3T3-L1predipocytes, the expression of PPAR γ2mRNA increased on different level. It demonstrated that insulin had promotion effect in cell differentiation. In this experiment, the expression of PPAR y2mRNA was growingly stronger when the concentration of insulin was increased.2. In vitro, the promotion effect of human insulin in promoting the differenentiation in3T3-L1adipocytes was the strongest, that of glargine insulin was weaker, that of detemir insulin was the weakest.