The Respiratory Movement Effects Of Electric Stimulation On Insular Cotrex Of Rats

Sleep apnea syndrome, also known as sleep apnea-hypopnea syndrome isapnoea hypopnea happen repeatedly more than30times per night in7hours orsleep apnea-hypopnea index (AHI)≥5/hr and accompanied by lethargy andother symptoms.Line polysomnography instrument (PSG) monitoring canconfirm the diagnosis. According to the pathogenesis the disease is divided intothree categories. Obstructive sleep apnea syndrome are more than the other twotypes. When suffering from obstructive sleep apnea syndrome (OSA), due torecurrent episodes of hypoxemia, hypercapnia can cause neurological disorders,catecholamines, endothelin and renin-angiotensin system disorders, endocrinedysfunction and blooddynamics change,the disease can result in multiple organsystem damage. Human health is affected seriously. Epidemiologyinvestigation for this disease in many areas of China shows that the mortalityrate was about4%, and in male is higher than in the female.Due to theimprovement of living standard and the change of people’s living habits, theincidence of the disease is rising, the study of the central mechanisms of thedisease and possible treatments without delay.Objective：To investigate the respiratory movement effects of electricstimulation on insular cortex of rats. And On this basis, provides the theorybasis for further research on the pathogenesis of OSAMethods: The adult Wistar rats were (weight220-250g, male and femaleunlimited) selected for the experiments. Experiments were performed on ratsby electrical stimulation with electrode at the INS and Hb, recording the changeof respiratory frequency and myoelectricity of genioglossus muscle anddiaphragmatic muscle. Use SPSS13.0soft ware to conduct the datum. The continuous variables were reprensented by mean±standarddeviation.Compares any two groups of t-test, P <0.05for significantdifference.Results：1.The respiratory effects and the changes of myoelectricity ofgenioglossus muscle and diaphragmatic muscle by electrical stimulation on theinsula（INS）: The experimental group showed that the natural breathing patternof the rats increased in tempo and depth.The myoelectricity of genioglossusmuscle was inhibited and myoelectricity of diaphragmatic muscle wasstrengthened. The control group showed no obvious change in frequency andbreadth by electrical stimulation on the INS. The myoelectricity ofgenioglossus muscle and diaphragmatic muscle had also no change.2.Theresult of stimulating on the Hb was the same to stimulating on the Hb.3.Highfrequency（200Hz） stimulation on the Hb can not induce the changes of themyoelectricity of genioglossus muscle and diaphragmatic muscle.Conclusion:1. The respiratory effects and myoelectricity of genioglossusmuscle and diaphragmatic muscle of rats can be changed by electricalstimulation on the INS.2. The respiratory effects and myoelectricity ofgenioglossus muscle and diaphragmatic muscle of rats can be changed byelectrical stimulation on the Hb.3. Different frequency electrical stimulationhave different effects on respiratory effects of rat, high frequency electricstimulation may block theHb, which block signaling of INS.