Infuence Of The Influenza A(H1N1)Virus In Organism Immune Response

Background:The novel swine origin2009influenza A (H1N1) virus was identified in April2009, and it is currently causing the first influenza pandemic of the21st century. Review of the last century, there were three global influenza pandemics have resulted in large-scale plague and a lot of deaths.(1)1918to1919Spanish flu (H1N1) pandemic, resulting in40to50million deaths;(2)1957to1958Asian flu (H2N2) pandemic killed nearly2million deaths;(3)1968to1969Hong Kong flu (H1N1) pandemic killed about1million deaths; The new virus has gene segments from the North American triple-reassortant and Eurasian swine H1N1viruses. Sequence analysis of this new pandemic virus revealed that hemagglutinin (HA), NP, and NS gene segments are derived from the classical swine viruses, PB1from human H3N2, and PB2and PA from avian viruses within the triple-reassortant virus.The virus is a completely new reassortant virus. The pandemic2009(H1N1) virus is genetically and antigenically distinct from previous seasonal human influenza A (H1N1) viruses. Thus, the current seasonal influenza vaccines are likely to give little, if any, protection against pandemic2009A (H1N1) virus infection. In most cases,the pandemic2009A (H1N1) virus causes an uncomplicated respiratory tract illness with symptoms, fever, cough, body aches, headaches and other respiratory symptoms as same as those caused by seasonal influenza viruses; and some will appear gastrointestinal discomfort such as diarrhea and vomiting; Patients with severe will be secondary to pneumonia and respiratory failure and even death. When the body is infected by the influenza virus, the innate immune response and adaptive immune responses in controlling influenza virus infections both play an important role.To assess the damage on the immune response that influenza A(H1N1) virus caused, We need more in-depth understanding the effect that influenza A (H1N1) virus infection on innate immune response and adaptive immune responsesof the body.Objective:In this paper we will through observed the changes of NK, B,CD4+T,CD8+T cells and the body secrete antiviral cytokines in the absolute number in peripheral blood to assss the effect on the immune response when influenza A (H1N1) virus infected. The results of this study will contribute to a better understanding of the influenza A (H1N1) virus on the immune response of the body, and provide a direction to improve the anti-influenza A (HIN1) virus vaccine.Methods:Collected68patients[26patients with flu-like syndrome,29patients with mild influenza A (H1N1),13patients with severe influenza A (H1N1)], and20healths as control; Meet the above requirements refer to the individual, the morning fasting serum samples were collected5ML, which2ML injection (EDTA-K2) in the vacuum blood anticoagulant, immediately blood cell count; remaining3ML into the vacuum blood without anticoagulant, centrifugation, separation of non-Hemolytic serum,-70℃preservation. Used blood analyzer to counts blood cells; B%、CD4+T%、CD8+T%and NK%were dected by flow cytometry(FCM); the levels of TNF-α、IL-12、IL-18in serum were measured by enzyme-linked immunosorbent assay adsorption (ELISA).Results:1. Compared to the healthy control group, the absolute number of B、CD4+T、 CD8+T cells in flu-like syndrome group、mild influenza A (H1N1)group and severe influenza A (H1N1)group, decline in the different patient groups were significant (P <0.05).2. NK increase in flu-like syndrome group and mild influenza A (H1N1)group were significant (P<0.05); NK cell have no significant differences between mild influenza A (H1N1)group and the control.3. Compared to the healthy control group, the serum TNF-a, IL-12, IL-18levels in flu-like syndrome group、mild influenza A (H1N1) group and severe influenza A (H1N1) group were higher than those of the healthy control group(P<0.05).4. While there were no significant differences among the flu-like syndrome group, mild influenza A (H1N1) group, severe influenza A (HlNl)group.Conclusion:In peripheral blood the levels of TNF-α、IL-12、IL-18increase, NK cells increase but CD4+T、CD8+T cells decrease, after infected by influenza A (H1N1) virus.