Study On Cell Adhesion And Antibiosis Of PEG Hydrogel Gratfed Active Peptides

This article is directed at the question of surface functionalization on biological hydrogel, wegraft bioactive peptides onto the surface of PEG hydrogel by photopolymerization in order to increase itscompatibility and antibiosis about surface of cell. To graft cell adhesion molecule RGDS and antimicrobialbacitracin,respectively. Then to test compatibility of mouse fibroblast and antibiosis of Staphylococcusaureus on surface functionalized hydrogel. The mainly work of this study are:(1) Separating fibroblast and neuron from Kunming mouse and then culture,passage,freeze andrecovery them.This step is finished to provide cell resource for modified PEG hydrogel to proceed celladhesion experiment.(2) We use the pipe disc method to test its sensitivity property of protein bacitracin, fourGram-positive bacteriaon in our laboratory have been used in this experiment.And then selecting thesignificantly antibacterial one from the strains,which is staphylococcus aureus,it could be used as the teststrain for bacitracin grafted PEG hydrogel film.(3) Use PEGDA with a double bond at each end of different molecular weights as raw material,prepare a series of different physical properties of PEGDA hydrogel film by using of the UVpolymerization technology. And test the absorbent swelling and other physical parameters of PEGDAhydrogel films.(4) According to the experimental design we manufacture the transfer reagentACRL-PEG-RGDS.Taking advantage of this reagent and UV polymerization technology I grafted RGDSpeptide to the PEGDA hydrogel films,it makes modified PEGDA hydrogel films have higher cell adhesionability.To test cell activity of the modified PEGDA hydrogel film by MTT assay.(5) On the basis of the experimental design we devise the transfer reagentACRL-PEG-bacitracin.We use this reagent and UV polymerization technology to graft bacitracin toPEGDA hydrogel films,so modified PEGDA hydrogel film has antibiosis ability.Through testing bacterialactivity on PEGDA hydrogel films by MTT method.(6) We use the principle that fluorescamine and primary amino groups of peptides have fluorescent color reaction to test whether polypeptide RGDS and bacitracin grafted on PEGDA hydrogelfilms successfully.By watching Fluorescence microscopy, we can prove it worked.(7) We take advantages of the transfer reagent and UV polymerization technology to graft RGDSand bacitracin to PEGDA hydrogel films at the same time. To test the bio-activities of bacterium and cell onPEGDA hydrogel films by MTT method, the modified PEG hydrogel film has the ability of cell adhesionand antibiosis.