| Objective: To study the expression and the effect of Foxj1in the central nervoussystem in Traumatic Brain Injury.Methods: Healthy male Sprague-Dawley rats were randomly divided into3groups:operation group, sham operation group, and control group. The brain stab-injury models ofrat were performed. Western blot and immunohistochemistry analysis were showed toinvestigate the expression of Foxj1at various times after injury. Doubleimmunofluorescence staining was performed to reveal the cell location of Foxj1and therole of Foxj1in cellular proliferation after brain injury.Results: Western blot and immunohistochemistry analysis showed that the expressionof Foxj1gradually increased, reached a peak at day3after Traumatic Brain Injury, anddeclined during the following days. Double immunofluorescence staining revealed thatFoxj1was co-expressed with MAP-2and GFAP. In addition, we detected that Ki67hadthe co-localization with NeuN, GFAP and Foxj1.Conclusions: Foxj1may be involved in the pathophysiological and biochemicalprogression after Traumatic Brain Injury, which is associated with the outcome of brainfunction and neurogenesis induced by injury. |
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