HSP9017-DMAG Inhibitors Affect Colon Cancer SW620Cell Proliferation: An Experimental Study

Background:Colon cancer is a common malignant tumor, which seriously threat to human life andhealth. The traditional surgical operation and chemotherapy or radiotherapy methods havetheir limitations, we need to find new ways to treat colon cancer. Studies show that heatshock protein90(HSP90) is an important intracellular molecular chaperones, involved invarious protein folding, assembly and transportation.HSP90plays an important role ontumorigenesis and development and it has become a new target for tumor therapy. Westudied the effects of17-DMAG on human colon cancer cell line SW620in our research inorder to find a new way to treat colon cancer．Objective：Study of HSP90novel inhibitor17-DMAG on colon cancer cell line SW620proliferation and apoptosis, and the mechanism to make preliminary discuss.Methods：With different concentrations of17-DMAG on growth period of the human coloncancer SW620cells, apoptotic morphological changes are observed under microscope;application of CCK8method to detect the absorbance values, and calculates the cellgrowth inhibition rate; using flow cytometric analysis of cell cycle changes of each group;Annexin V-FITC and PI double staining method for detecting clusters of apoptotic cellsrate of change.Results：We observed through microscope that17-DMAG drug treatment group cells appearedapoptosis. CCK8assay suggested that17-DMAG inhibited the proliferation of humancolon cancer cell line SW620in a dose-and time-dependent manner(P<0.05)．Annexin-FITC/PI assay revealed that cell apoptotic ratios of1.0umol/L、2.5umol/L、5.0umol/Ldensity were30.03%、50.50%and77.23%,The difference was significant compared to the control group (P<0.05). Flow cytometric cell cycle analysis showed that the17-DMAGgroups treated with different concentrations of the drug compared with the control group,the G0/G1cell ratio increased significantly, S cell ratio decreased, G2/M cell ratioincreased significantly, P<0.05, with statistical difference; but the concentration of17-DMAG drug groups comparison of G0/G1, S, G2/M period, the cell ratio did notchange significantly, the differences were not statistically significant (P>0.05).Conclusion:HSP9017-DMAG inhibitor on colon cancer cell SW620proliferation is inhibited in adose-and time-dependent, and induce its apoptosis; at the same time, the17-DMAG effectof SW620cell cycle, but this effect is independent of the concentration of17-DMAG..